The complete sequences of African horsesickness virus serotype 4 (vaccine strain) RNA segment 2 and 6 which encode outer capsid protein
| Autor: | Takeshi Tsuchiya, Masanobu Kamada, Akio Fukusho, Hiroshi Imagawa, Takeo Sugiura, Susumu Ueda, Akira Iwata, Noriko Mizukoshi, Kenichi Sakamoto, Bunchong Apiwatnakorn |
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| Rok vydání: | 1994 |
| Předmět: |
Molecular Sequence Data
Vaccines Attenuated Conserved sequence Capsid Complementary DNA Sequence Homology Nucleic Acid African Horse Sickness Virus Animals Amino Acid Sequence Serotyping Codon Peptide sequence Vero Cells Conserved Sequence RNA Double-Stranded chemistry.chemical_classification Orbivirus General Veterinary biology Base Sequence Sequence Homology Amino Acid Nucleic acid sequence RNA Viral Vaccines biology.organism_classification Virology Amino acid Open reading frame chemistry RNA Viral |
| Zdroj: | The Journal of veterinary medical science. 56(2) |
| ISSN: | 0916-7250 |
| Popis: | The complete sequences of RNA segment 2 and segment 6 of African horsesickness virus serotype 4 (AHSV-4) vaccine strain were determined from cDNA clones inserted into pBR 322. The RNAs of segment 2 and 6 are 3229, 1566 bp long respectively and both contain an open reading frame encoding proteins VP2 and VP5 of 1060, 505 amino acid residues. The estimated molecular weight of VP2 was 124,178 dalton and that of VP5 was 56,793 dalton. Their noncoding end sequences were 5'GTTTAA . . . and . . . ACATAC3' (segment 2), 5'GTTTAT . . . and . . . ACTTAC3' (segment 6). They were different from orbivirus characteristic terminal sequences, which were 5'GTTAAA . . . and . . . ACTTAC3'. The comparison of both sequences of AHSV-4 segment 2 and 6 with those of segment 2 and 5 of bluetongue virus (BTV) serotype 10 revealed 53% nucleotide similarity and 23% amino acid similarity (segment 2), and 58% nucleotide similarity and 46% amino acid similarity (segment 6). In the same way, the comparison of both sequences of the vaccine strain with those of the virulent strain segment 2 and segment 6 of AHSV-4 revealed 91% nucleotide and 96% amino acid similarity (segment 2), and 98% nucleotide and 98% amino acid similarity (segment 6). |
| Databáze: | OpenAIRE |
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