Sex- and Cell-Specific Expression of an Estrogen Receptor Isoform in the Pituitary Gland
| Autor: | Florence Demay, Christophe Tiffoche, Marie-Lise Thieulant |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Male
Pituitary gland medicine.medical_specialty Endocrinology Diabetes and Metabolism Estrogen receptor Biology Gonadotropic cell Prolactin cell Cellular and Molecular Neuroscience Sex Factors Endocrinology Internal medicine medicine Animals RNA Messenger Rats Wistar Sex Distribution Estrogen receptor beta Messenger RNA Endocrine and Autonomic Systems Alternative splicing Blotting Northern Rats medicine.anatomical_structure Receptors Estrogen Pituitary Gland Female Estrogen receptor alpha |
| Zdroj: | Neuroendocrinology. 63:522-529 |
| ISSN: | 1423-0194 0028-3835 |
| DOI: | 10.1159/000127081 |
| Popis: | The presence of multiple monomeric forms has been described for the estrogen receptor (ER) in the pituitary gland. We analyzed ER mRNA forms in male and female rat pituitary. A single 6.2-kb ER mRNA species was detected in the male rat pituitary, whereas the female rat pituitary exhibited two ER mRNA forms of 6.2 and 5.5 kb, respectively. The 6.2-kb mRNA was present throughout the different stages of the estrous cycle, while the 5.5-kb mRNA appeared to be restricted to proestrus, suggesting an acute regulation of ER transcription at this stage. The 5.5-kb ER mRNA could be rapidly induced either by 17 beta-estradiol replacement in ovariectomized adult female rats or by priming immature rats with pregnant-mare serum gonadotropin. Using enriched cell populations, an inverse and strong correlation was established between the presence of the 5.5-kb ER mRNA form and the number of gonadotropes. Conversely, the localization of the 5.5-kb mRNA form was demonstrated in lactotrope populations. In order to elucidate the structural modifications in the transiently expressed ER mRNA, a series of reverse-transcriptase polymerase chain reaction amplifications was carried out using several pairs of primers corresponding to the entire ER-coding region. The data showed that no alternative splicing was occurring in the ER-coding region involving a potential role of either 3'- or 5'-untranslated regions. Thus, ER presents a 17 beta-estradiol-dependent transcriptional mechanism triggered on proestrous day and specific to the female lactotropes. |
| Databáze: | OpenAIRE |
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