Prevalence of PKD1gene mutation in cats in Turkey and pathogenesis of feline polycystic kidney disease
| Autor: | Sevim Isparta, Yusuf Şen, Bengi Çinar Kul, Özge Şebnem Çildir, Zeynep Bars, Nuket Bilgen, Merve Bişkin Türkmen, Mustafa Yenal Akkurt |
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| Přispěvatelé: | KKÜ |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
kidney
040301 veterinary sciences Fibrocystin Physiology Gene mutation Asymptomatic 0403 veterinary science 03 medical and health sciences autosomal dominant Polycystic kidney disease Medicine 030304 developmental biology ADPKD 0303 health sciences Kidney CATS polycystic kidney disease General Veterinary biology PKD1 business.industry cats 04 agricultural and veterinary sciences medicine.disease hereditary disease medicine.anatomical_structure Hereditary Diseases biology.protein medicine.symptom business |
| Popis: | Isparta, Sevim/0000-0002-1575-7861; Bars, Zeynep/0000-0002-8116-1855; CINAR KUL, Bengi/0000-0002-8955-0097; Cildir, Ozge Sebnem/0000-0001-7070-4212 WOS:000550747800007 PubMed: 32687010 Polycystic kidney disease (PKD) is one of the most common hereditary diseases in cats, with high prevalence in Persian and Persian-related cats. PKD is caused mainly by an inherited autosomal dominant (AD) mutation, and animals may be asymptomatic for years. We screened 16 cats from various breeds exhibiting a renal abnormality by ultrasound examination and genotyped them for the c.10063C>A transversion on exon 29 of the polycystin-1 (PKD1) gene, by PCR-restriction fragment length polymorphism (PCR-RFLP). Among these cats, a Siamese nuclear family of 4 cats with ancestral hereditary renal failure were screened by whole-genome sequencing (WGS) to determine novel variations in genes associated with both AD and autosomal recessive PKD in humans. During the study period, one cat died as a result of renal failure and was forwarded for autopsy. Additionally, we screened 294 cats asymptomatic for renal disease (Angora, Van, Persian, Siamese, Scottish Fold, Exotic Shorthair, British Shorthair, and mixed breeds) to determine the prevalence of the mutation in cats in Turkey. Ten of the symptomatic and 2 of the asymptomatic cats carried the heterozygous C -> A transversion, indicating a prevalence of 62.5% and 0.68%, respectively. In the WGS analysis of 4 cats in the Siamese nuclear family, novel variations were determined in the fibrocystin gene (PKHD1), which was not compatible with dominant inheritance of PKD. |
| Databáze: | OpenAIRE |
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