Trace determination of metal ion inhibitors of the glucose-glucose oxidase system
| Autor: | E. Clifford Toren, F. Joseph Burger |
|---|---|
| Rok vydání: | 1968 |
| Předmět: |
Silver
Inorganic chemistry chemistry.chemical_element Maximum error Horseradish peroxidase Analytical Chemistry Metal Glucose Oxidase chemistry.chemical_compound Methods Glucose oxidase Hydrogen peroxide chemistry.chemical_classification biology Mercury Peroxides Mercury (element) Glucose Enzyme Lead Peroxidases chemistry Spectrophotometry visual_art Gluconic acid biology.protein visual_art.visual_art_medium Nuclear chemistry |
| Zdroj: | Microchimica Acta. 56:538-545 |
| ISSN: | 1436-5073 0026-3672 |
| DOI: | 10.1007/bf01224060 |
| Popis: | The inhibition of the glucose oxidase enzyme by three heavy metal inhibitors, Ag(I), Hg(II), and Pb(II) is reported. The method for the microdetermination of these metals is based on the decreased rate of the enzyme-catalyzed, aerobic oxidation of glucose to gluconic acid and hydrogen peroxide. In the presence of horseradish peroxidase, the rapid oxidation ofo-dianisidine by hydrogen peroxide can be followed spectrophotometrically at 440 nm. Silver(I) has been determined in the range of 0.005 to 0.2μg/ml with an absolute error of less than 14 ng. Mercury(II) has been determined in the range of 0.1 to 0.4μg/ml with a maximum error of 34 ng. Below a concentration of 260μg/ml Pb(II), no appreciable inhibition was observed. Results indicate the utility of enzymatic methods to the trace determination of metal ion inhibitors. |
| Databáze: | OpenAIRE |
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