An HLA-DR/DP recombinant family involving DPw6. Evidence for cross-reactivity between DPw6 and GNN2B
| Autor: | Robert J. Hartzman, Fu-Meei Robbins, Arne Svejgaard, Steven Shaw, Per Platz, Lars P. Ryder, Niels Ødum, Bodil K. Jakobsen, Niels Morling |
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| Rok vydání: | 2008 |
| Předmět: |
Male
HLA-DP Antigens Genetic Linkage T-Lymphocytes Genes MHC Class II Immunology Human leukocyte antigen Immunogenetics Cross Reactions Biology medicine.disease_cause Biochemistry Cross-reactivity law.invention Antigen HLA Antigens law Freezing Genetics HLA-DR medicine Humans Immunology and Allergy Typing Child Gene Alleles Cells Cultured Recombination Genetic Histocompatibility Antigens Class II HLA-DR Antigens General Medicine Recombinant DNA Female |
| Zdroj: | University of Copenhagen |
| ISSN: | 1399-0039 0001-2815 |
| DOI: | 10.1111/j.1399-0039.1986.tb01496.x |
| Popis: | HLA typing of an HLA-B/GLO recombinant family using bulk-expanded DP(SB) alloreactive T-cells (GNN1 to 6) as well as conventional HLA-ABC, D, DR. and DQ typing showed that a paternal recombination had taken place between HLA-DR and DQ on the one hand and HLA-DP(SB) on the other. The recombinant child was DPw4, 6-heterozygous and differed in terms of class II determinants only for DPw6 from two otherwise HLA identical siblings, who were probably DPw4/4-homozygous. These siblings did not stimulate the recombinant in MLC whereas they responded to his cells indicating that DPw6 can stimulate in primary MLC. Moreover, it was possible to generate and bulk-expand DPw6-reactive lymphocytes (PLs) between MT and MA as responders and BN as stimulator. The correlation coefficient (r) between the reaction of these PLs and the GNN6 (anti-DPw6) reagents was 1.0 when tested against a panel of 71 individuals. This is the first report demonstrating that the DPw6 gene, like the DPw1-w5 genes, is located between DR/DQ and GLO. The frequency of the DPw6 antigen in 41 unrelated, randomly selected Danes was 7%. HLA-DP typing of the BN family revealed that the DPw6 positive but not DPw6 negative family members gave intermediate responses with one (GNN2B) but not with another (GNN2A) anti-DPw2-reagent. Studies of 63 healthy individuals (unrelated to the BN family) revealed seven similar discrepancies between GNN2A and GNN2B. Strikingly, all four DPw6 positive stimulators gave rise to such discrepancies indicating that the GNN2B reagent is cross-reactive and recognize a common part of the DPw2 and DPw6 molecules. No cases of GNN2A+/GNN2B- stimulators were found. |
| Databáze: | OpenAIRE |
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