Toward an accessible and robust in vitro approach to evaluate bacterial viability in the upper gastro-intestinal tract: A Gastro-Intestinal Digestive Simulator (GIDS) combined with alternative methods to plating
| Autor: | Takuya Akiyama, Sophie Legrain-Raspaud, Nadège Adouard, Kaihei Oki, Yasmina Belaïnoussi, Lolita Maufoux, Rémi Brazeilles, Christèle Tison, Kenji Oishi, Raphael Moriez, Junji Fujimoto, Biliana Lesic |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Lactobacillus paracasei ved/biology.organism_classification_rank.species Medicine (miscellaneous) Biology 03 medical and health sciences 0404 agricultural biotechnology Fermented milk products TX341-641 Flow cytometry Simulation Alternative methods 030109 nutrition & dietetics Nutrition and Dietetics Bifidobacterium breve Gastro-intestinal in vitro model Bacteria Nutrition. Foods and food supply ved/biology PMA-qPCR 04 agricultural and veterinary sciences biology.organism_classification 040401 food science In vitro Bifidobacterium animalis Viability Digestive tract Bacterial Viability Food Science |
| Zdroj: | Journal of Functional Foods, Vol 59, Iss, Pp 30-39 (2019) |
| ISSN: | 1756-4646 |
| Popis: | Since the viability throughout the human gastro-intestinal (GI) tract is a key metric to select new probiotics, we aimed to develop an evolutive and robust semi-dynamic model, named Gastro-Intestinal Digestive Simulator (GIDS), mimicking pH variations of the upper GI tract and based on the latest Infogest in vitro digestion protocol recommendations. We first tested the GIDS and its reproducibility by measuring, during in vitro digestion, the cultivability of bacterial strain contained in two marketed fermented milk products. Then, we used this model to assess inter- and intra-species variability among 16 bacterial strains from Bifidobacterium animalis, Bifidobacterium breve, and Lactobacillus paracasei species. Finally, we further explored the applicability of propidium monoazide-quantitative PCR and flow cytometry to GIDS samples. To summarize, our study showed that i) the GIDS model is reproducible and ii) the complementary methods to plating tested here are compatible with GI environments; providing insights that will guide future developments. |
| Databáze: | OpenAIRE |
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