Orthogonal lipid sensors identify transbilayer asymmetry of plasma membrane cholesterol
| Autor: | Shu-Lin Liu, Irena Levitan, Ewa Stec, Wonhwa Cho, Jae Hun Jung, Li Wang, Seohyoen Song, Robert A. Winn, Rama Kamesh Bikkavilli, Matthew J. O'Connor, Kazumitsu Ueda, Daesung Lee, Kwang Pyo Kim, Kwanghee Baek, Ren Sheng |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cell signaling Cell Lipid Bilayers plasma membrane Article Cell Line Cell membrane 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine None medicine Humans Animals Molecular Biology Membranes Chemistry Cholesterol HEK 293 cells Cell Membrane Wnt signaling pathway Cell Biology Lipids orthogonal lipid sensors Cell biology HEK293 Cells 030104 developmental biology medicine.anatomical_structure Membrane Liposomes lipids (amino acids peptides and proteins) transbilayer asymmetry Scientific Correspondence 030217 neurology & neurosurgery Homeostasis |
| Zdroj: | eLife |
| Popis: | The plasma membrane in mammalian cells is rich in cholesterol, but how the cholesterol is partitioned between the two leaflets of the plasma membrane remains a matter of debate. Recently, Liu et al. used domain 4 (D4) of perfringolysin O as a cholesterol sensor to argue that cholesterol is mostly in the exofacial leaflet (Liu et al., 2017). This conclusion was made by interpreting D4 binding in live cells using in vitro calibrations with liposomes. However, liposomes may be unfaithful in mimicking the plasma membrane, as we demonstrate here. Also, D4 binding is highly sensitive to the presence of cytosolic proteins. In addition, we find that a D4 variant, which requires >35 mol% cholesterol to bind to liposomes in vitro, does in fact bind to the cytoplasmic leaflet of the plasma membrane in a cholesterol-dependent manner. Thus, we believe, based on the current evidence, that it is unlikely that there is a significantly higher proportion of cholesterol in the exofacial leaflet of the plasma membrane compared to the cytosolic leaflet. |
| Databáze: | OpenAIRE |
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