Trichloroethylene exposure in mid-pregnancy decreased fetal weight and increased placental markers of oxidative stress in rats
| Autor: | Anjana Kumar, Lawrence H. Lash, Rita Loch-Caruso, Iman Hassan, Sean M. Harris, Faith Bjork |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Trichloroethylene
Placenta Birth weight 010501 environmental sciences Toxicology medicine.disease_cause 01 natural sciences Article Dioxygenases Andrology 03 medical and health sciences chemistry.chemical_compound Pregnancy medicine Animals Rats Wistar Maternal-Fetal Exchange 030304 developmental biology 0105 earth and related environmental sciences 0303 health sciences biology business.industry Deoxyguanosine Glutathione Oxidative Stress Glutathione S-transferase medicine.anatomical_structure Fetal Weight chemistry 8-Hydroxy-2'-Deoxyguanosine embryonic structures 5-Methylcytosine Solvents biology.protein Immunohistochemistry Gestation Female business Biomarkers Oxidative stress |
| Zdroj: | Reproductive Toxicology. 83:38-45 |
| ISSN: | 0890-6238 |
| DOI: | 10.1016/j.reprotox.2018.11.002 |
| Popis: | Although epidemiology studies have associated maternal trichloroethylene (TCE) exposure with decreased birth weight and preterm birth, mechanistic explanations for these associations are currently lacking. We hypothesized that TCE targets the placenta with adverse consequences for pregnancy outcomes. Pregnant Wistar rats were exposed orally to vehicle or 480 mg TCE/kg body weight from gestational days (gd) 6–16, and tissues were collected on gd 16. Exposure to TCE significantly decreased average fetal weight without reducing maternal weight. In placenta, TCE significantly increased 8-hydroxy-deoxyguanosine, global 5-hydroxymethylcytosine, and mRNA expression of Tet3, which codes for an enzyme involved in 5-hydroxymethylcytosine formation. Furthermore, glutathione S-transferase activity and immunohistochemical staining were increased in placentas of TCE-exposed rats. The present study provides the first evidence that TCE increases markers of oxidative stress in placenta in a fetal growth restriction rat model, providing new insight into the placenta as a potentially relevant target for TCE-induced adverse pregnancy outcomes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect