Toxicity screening of a Gambierdiscus australes strain from the Western Mediterranean sea and identification of a novel maitotoxin analogue
| Autor: | Philipp Hess, José Manuel Leão-Martins, Manoella Sibat, Ana Gago-Martínez, Jorge Diogène, Robert W. Dickey, Pablo Estevez, David Castro, Angels Tudó |
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| Přispěvatelé: | Producció Animal, Aigües Marines i Continentals |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Aquatic Organisms
LC-HRMS Ciguatera Ciguatoxin QH301-705.5 3206.11 Toxicidad de Los Alimentos Pharmaceutical Science Zoology 010402 general chemistry 01 natural sciences Article Gambierdiscus australes ciguatera poisoning Structure-Activity Relationship chemistry.chemical_compound Mediterranean sea Drug Discovery Mediterranean Sea medicine Animals 14. Life underwater Biology (General) LC-MS/MS Pharmacology Toxicology and Pharmaceutics (miscellaneous) 3105 Peces y Fauna Silvestre Maitotoxin Strain (chemistry) biology Oxocins 010401 analytical chemistry maitotoxin Dinoflagellate MS biology.organism_classification medicine.disease LC-MS 0104 chemical sciences N2a 2302.90 Bioquímica de Alimentos chemistry Toxicity Dinoflagellida Marine Toxins ciguatoxin |
| Zdroj: | Marine Drugs Volume 19 Issue 8 Marine Drugs, Vol 19, Iss 460, p 460 (2021) IRTA Pubpro. Open Digital Archive Institut de Recerca i Tecnologia Agroalimentàries (IRTA) Investigo. Repositorio Institucional de la Universidade de Vigo Universidade de Vigo (UVigo) Marine Drugs (1660-3397) (MDPI AG), 2021-08, Vol. 19, N. 8, P. 460 (19p.) |
| Popis: | Dinoflagellate species of the genera Gambierdiscus and Fukuyoa are known to produce ciguatera poisoning-associated toxic compounds, such as ciguatoxins, or other toxins, such as maitotoxins. However, many species and strains remain poorly characterized in areas where they were recently identified, such as the western Mediterranean Sea. In previous studies carried out by our research group, a G. australes strain from the Balearic Islands (Mediterranean Sea) presenting MTX-like activity was characterized by LC-MS/MS and LC-HRMS detecting 44-methyl gambierone and gambieric acids C and D. However, MTX1, which is typically found in some G. australes strains from the Pacific Ocean, was not detected. Therefore, this study focuses on the identification of the compound responsible for the MTX-like toxicity in this strain. The G. australes strain was characterized not only using LC-MS instruments but also N2a-guided HPLC fractionation. Following this approach, several toxic compounds were identified in three fractions by LC-MS/MS and HRMS. A novel MTX analogue, named MTX5, was identified in the most toxic fraction, and 44-methyl gambierone and gambieric acids C and D contributed to the toxicity observed in other fractions of this strain. Thus, G. australes from the Mediterranean Sea produces MTX5 instead of MTX1 in contrast to some strains of the same species from the Pacific Ocean. No CTX precursors were detected, reinforcing the complexity of the identification of CTXs precursors in these regions. European Food Safety Authority | Ref. GP/EFSA/AFSCO/2015/03 Xunta de Galicia | Ref. ED481A-2018/207 Generalitat de Catalunya | Ref. CERCA |
| Databáze: | OpenAIRE |
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