Diet intervention reduces uptake of αvβ3 integrin-targeted PET tracer 18F-galacto-RGD in mouse atherosclerotic plaques
| Autor: | Iina Laitinen, Seppo Ylä-Herttuala, Juhani Knuuti, Axel Walch, Gabriele Hölzlwimmer, Stephan G. Nekolla, Irina Lisinen, Irene Esposito, Antti Saraste, Hans-Jürgen Wester, Peter B. Luppa, Moritz Wildgruber, Eliane Weidl, Axel W. Weber, Markus Schwaiger, Pia Leppänen |
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| Rok vydání: | 2011 |
| Předmět: |
Male
Pathology medicine.medical_specialty Transgene Hypercholesterolemia Inflammation Mice Transgenic medicine.disease_cause Diet High-Fat Peptides Cyclic Lesion Mice medicine Animals Humans Radiology Nuclear Medicine and imaging Pet tracer Apolipoproteins B αvβ3 integrin medicine.diagnostic_test business.industry Macrophages Galactose Flow Cytometry Integrin alphaVbeta3 Vulnerable plaque Animal Feed Plaque Atherosclerotic Mice Inbred C57BL Cholesterol Positron emission tomography Positron-Emission Tomography 18F-Galacto-RGD Autoradiography medicine.symptom Radiopharmaceuticals Cardiology and Cardiovascular Medicine business |
| Zdroj: | Journal of nuclear cardiology : official publication of the American Society of Nuclear Cardiology. 19(4) |
| ISSN: | 1532-6551 |
| Popis: | Expression of α(v)β(3) integrin has been proposed as a marker for atherosclerotic lesion inflammation. We studied whether diet intervention reduces uptake of α(v)β(3) integrin-targeted positron emission tomography tracer (18)F-galacto-RGD in mouse atherosclerotic plaques.Hypercholesterolemic LDLR(-/-) ApoB(100/100) mice on high-fat diet for 4 months were randomized to further 3 months on high-fat diet (high-fat group, n = 8) or regular mouse chow (intervention group, n = 7). Intima-media ratio describing plaque burden was comparable between intervention and high-fat groups (2.0 ± 0.5 vs 2.3 ± 0.8, P = .5). Uptake of (18)F-galacto-RGD in the aorta was lower in the intervention than high-fat group (%ID/g 0.16 vs 0.23, P.01). Autoradiography showed 35% lower uptake of (18)F-galacto-RGD in the atherosclerotic plaques in the intervention than high-fat group (P = .007). Uptake of (18)F-galacto-RGD in plaques correlated with uptake of (3)H-deoxyglucose and nuclear density, which was lower in the intervention than high-fat group (P = .01). Flow cytometry demonstrated macrophages expressing α(v) and β(3) integrins in the aorta.Uptake of (18)F-galacto-RGD in mouse atherosclerotic lesions was reduced by lipid-lowering diet intervention. Expression of α(v)β(3) integrin is a potential target for evaluation of therapy response in atherosclerosis. |
| Databáze: | OpenAIRE |
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