Meiotic recombination analyses in pigs carrying different balanced structural chromosomal rearrangements
| Autor: | Harmonie Barasc, Alain Ducos, Anne Calgaro, N. Bonnet, M Yerle, Aurélia Priet, Alain Pinton, Anne-Marie Loustau-Dudez, Nicolas Mary, Stéphane Ferchaud |
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| Přispěvatelé: | Génétique Physiologie et Systèmes d'Elevage (GenPhySE ), École nationale supérieure agronomique de Toulouse [ENSAT]-Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Génétique, Expérimentation et Système Innovants (GenESI), Institut National de la Recherche Agronomique (INRA), Université Fédérale Toulouse Midi-Pyrénées |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine DNA Repair Swine [SDV]Life Sciences [q-bio] Sus scrofa reciprocal translocation Gene Expression lcsh:Medicine Chromosomal translocation 030105 genetics & heredity Interference (genetic) Translocation Genetic Animal Cells Spermatocytes human oocyte DNA Breaks Double-Stranded Crossing Over Genetic lcsh:Science In Situ Hybridization Fluorescence ComputingMilieux_MISCELLANEOUS Chromosomal inversion Genetics Multidisciplinary Chromosome Biology Synapsis crossover interference Chromatin Chromosomal Aberrations Meiosis house mouse Translocations Epigenetics Cellular Types MutL Protein Homolog 1 Research Article Chromosome Structure and Function endocrine system crossing over Biology Chromosomes 03 medical and health sciences Homologous chromosome Animals synaptonemal complex analysis [INFO]Computer Science [cs] synapsis pattern [SDV.GEN]Life Sciences [q-bio]/Genetics lcsh:R Biology and Life Sciences Cell Biology Chromosome Pairs Sperm Chromosome 3 Chromosome 4 Chromosome Pairing [SDV.GEN.GA]Life Sciences [q-bio]/Genetics/Animal genetics Germ Cells 030104 developmental biology Chromosome Inversion lcsh:Q Homologous recombination Sister Chromatid Exchange |
| Zdroj: | PLoS ONE PLoS ONE, Public Library of Science, 2016, 11 (4), Non paginé. ⟨10.1371/journal.pone.0154635⟩ 22nd International Colloquium on Animal Cytogenetics and Genomics. (24 (S1))2016; 22. International Colloquium on Animal Cytogenetics and Genomics (ICACG), Toulouse, FRA, 2016-07-02-2016-07-05, S5-S6 Chromosome Research 22. International Colloquium on Animal Cytogenetics and Genomics (ICACG) 22. International Colloquium on Animal Cytogenetics and Genomics (ICACG), Jul 2016, Toulouse, France. 116 p Plos One 4 (11), Non paginé. (2016) PLoS ONE, Vol 11, Iss 4, p e0154635 (2016) |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0154635⟩ |
| Popis: | Correct pairing, synapsis and recombination between homologous chromosomes are essential for normal meiosis. All these events are strongly regulated, and our knowledge of the mechanisms involved in this regulation is increasing rapidly. Chromosomal rearrangements are known to disturb these processes. In the present paper, synapsis and recombination (number and distribution of MLH1 foci) were studied in three boars (Sus scrofa domestica) carrying different chromosomal rearrangements. One (T34he) was heterozygote for the t(3;4)(p1.3;q1.5) reciprocal translocation, one (T34ho) was homozygote for that translocation, while the third (T34Inv) was heterozygote for both the translocation and a pericentric inversion inv(4)(p1.4;q2.3). All three boars were normal for synapsis and sperm production. This particular situation allowed us to rigorously study the impact of rearrangements on recombination. Overall, the rearrangements induced only minor modifications of the number of MLH1 foci (per spermatocyte or per chromosome) and of the length of synaptonemal complexes for chromosomes 3 and 4. The distribution of MLH1 foci in T34he was comparable to that of the controls. Conversely, the distributions of MLH1 foci on chromosome 4 were strongly modified in boar T34Inv (lack of crossover in the heterosynaptic region of the quadrivalent, and crossover displaced to the chromosome extremities), and also in boar T34ho (two recombination peaks on the q-arms compared with one of higher magnitude in the controls). Analyses of boars T34he and T34Inv showed that the interference was propagated through the breakpoints. A different result was obtained for boar T34ho, in which the breakpoints (transition between SSC3 and SSC4 chromatin on the bivalents) seemed to alter the transmission of the interference signal. Our results suggest that the number of crossovers and crossover interference could be regulated by partially different mechanisms. |
| Databáze: | OpenAIRE |
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