Murine model to follow hyphal development in invasive pulmonary aspergillosis

Autor:	Zoltán Hargitai, István Pócsi, József Balla, László Csernoch, Zsuzsa M. Szigeti, Péter Szentesi, Laszlo Talas, Gaspar Banfalvi, Gábor Szemán-Nagy, Zoltán Palicz
Rok vydání:	2018
Předmět:	0301 basic medicine Cyclophosphamide Hypha medicine.medical_treatment 030106 microbiology Colony Count Microbial Hyphae Severity of Illness Index Applied Microbiology and Biotechnology Aspergillus fumigatus Microbiology Immunocompromised Host Mice 03 medical and health sciences In vivo medicine Animals Elméleti orvostudományok Survival rate Invasive Pulmonary Aspergillosis Aspergillus biology Histocytochemistry Inoculation business.industry Body Weight Immunosuppression Orvostudományok General Medicine biology.organism_classification Survival Analysis Disease Models Animal 030104 developmental biology business Biotechnology medicine.drug
Zdroj:	Applied Microbiology and Biotechnology. 102:2817-2825
ISSN:	1432-0614 0175-7598
DOI:	10.1007/s00253-018-8800-4
Popis:	Aspergillus fumigatus is an opportunistic pathogen, the leading cause of invasive and disseminated aspergillosis in systemic immunocompromised patients, and an important cause of mortality. The aim of the present study was to adapt a pulmonary aspergillosis murine model, to determine pathodynamical parameters quantitatively, and to follow the progression of fungal infection in vivo. The nasal inoculation of Aspergillus conidia in mice previously subjected to immunosuppression with cyclophosphamide (CP) turned out to be a more suitable model than that of immunosuppressed with hydrocortisone (HC). The following parameters were found to correlate quantitatively with the progress of the infection: (i) survival rate, (ii) weight loss of mice, (iii) infected focal plaque size, (iv) hyphal density, (v) hyphal length distribution of A. fumigatus, and the (vi) the histopathological status and scores. These parameters will be essential elements for the development of antifungal drugs and therapies, and important for the investigation of the pathogenicity in different strains of A. fumigatus.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::44d9f5e83bc7a094c8ffac3d57028db1 https://doi.org/10.1007/s00253-018-8800-4 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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