Analysis and Characterization of Staphylococcus aureus Small Colony Variants Isolated From Cystic Fibrosis Patients in Austria
| Autor: | Gebhard Feierl, Ernst Eber, Andrea J. Grisold, Clemens Kittinger, Tanja Kaschnigg, Lilian Masoud-Landgraf, Gernot Zarfel, Ute Wagner-Eibel, Simone Friedl |
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| Jazyk: | angličtina |
| Předmět: |
0301 basic medicine
Adult Male Staphylococcus aureus Adolescent Cystic Fibrosis Mucociliary clearance medicine.drug_class Auxotrophy 030106 microbiology Antibiotics Microbial Sensitivity Tests Biology medicine.disease_cause Staphylococcal infections Cystic fibrosis Applied Microbiology and Biotechnology Microbiology Article 03 medical and health sciences Young Adult medicine Humans Child Pseudomonas aeruginosa Infant General Medicine Middle Aged Staphylococcal Infections medicine.disease Anti-Bacterial Agents medicine.anatomical_structure Austria Child Preschool Female Respiratory tract |
| Zdroj: | Current Microbiology |
| ISSN: | 0343-8651 |
| DOI: | 10.1007/s00284-016-0994-z |
| Popis: | Cystic fibrosis (CF) is the most common hereditary lung disease in the Caucasian population, characterized by viscous bronchial secretion, consecutive defective mucociliary clearance, and unavoidable colonization with microorganisms. Besides Pseudomonas aeruginosa, Staphylococcus aureus is the most common bacterial species colonizing the CF respiratory tract. Under antibiotic pressure S. aureus is able to switch to small colony variants (SCV). These small colony variants can invade epithelial cells, overcome antibiotic therapy inside the cells and can be the starting point for extracellular recolonization. The aim of the present study was the isolation and characterization of S. aureus small colony variants from Austrian cystic fibrosis patients. Samples collected from 147 patients were screened for the presence of S. aureus wild-type and small colony variants. Antibiotic susceptibility testing and determination of the small colony variants causing auxotrophism were performed. Wild-type isolates were assigned to corresponding small colony variants with spa typing. In total, 17 different small colony variant isolates and 12 corresponding wild-type isolates were obtained. 13 isolates were determined thymidine auxotroph, 2 isolates were auxotroph for hemin, and none of the tested isolates was auxotroph for both, respectively. The presence of SCVs is directly related to a poor clinical outcome, therefore a monitoring of SCV prevalence is recommended. This study revealed rather low SCV ratios in CF patients compared to other countries. |
| Databáze: | OpenAIRE |
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