Endoplasmic Reticulum Stress and Autophagy Markers in Soleus Muscle Disuse-Induced Atrophy of Rats Treated with Fish Oil
| Autor: | Jorge Mancini-Filho, Tatiana Carolina Alba-Loureiro, Gilson Masahiro Murata, Kaio Fernando Vitzel, Gabriel Nasri Marzuca-Nassr, Wilson Mitsuo Tatagiba Kuwabara, Rosângela Pavan Torres, Rui Curi |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty Docosahexaenoic Acids p38 mitogen-activated protein kinases ATROFIA MUSCULAR ω-3 fatty acids Article 03 medical and health sciences Gastrocnemius muscle 0302 clinical medicine Atrophy skeletal muscle atrophy Fish Oils Internal medicine medicine Autophagy Animals TX341-641 Rats Wistar Muscle Skeletal Soleus muscle Nutrition and Dietetics Nutrition. Foods and food supply Chemistry Endoplasmic reticulum unfolded protein response docosahexaenoic acid Fish oil medicine.disease eicosapentanoic acid Endoplasmic Reticulum Stress Rats Disease Models Animal Muscular Atrophy 030104 developmental biology Endocrinology Eicosapentaenoic Acid Hindlimb Suspension Docosahexaenoic acid 030217 neurology & neurosurgery Biomarkers Food Science |
| Zdroj: | Nutrients Volume 13 Issue 7 Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP Nutrients, Vol 13, Iss 2298, p 2298 (2021) |
| ISSN: | 2072-6643 |
| Popis: | Endoplasmic reticulum stress (ERS) and autophagy pathways are implicated in disuse muscle atrophy. The effects of high eicosapentaenoic (EPA) or high docosahexaenoic (DHA) fish oils on soleus muscle ERS and autophagy markers were investigated in a rat hindlimb suspension (HS) atrophy model. Adult Wistar male rats received daily by gavage supplementation (0.3 mL per 100 g b.w.) of mineral oil or high EPA or high DHA fish oils (FOs) for two weeks. Afterward, the rats were subjected to HS and the respective treatments concomitantly for an additional two-week period. After four weeks, we evaluated ERS and autophagy markers in the soleus muscle. Results were analyzed using two-way analysis of variance (ANOVA) and Bonferroni post hoc test. Gastrocnemius muscle ω-6/ω-3 fatty acids (FAs) ratio was decreased by both FOs indicating the tissue incorporation of omega-3 fatty acids. HS altered (p < 0.05) the protein content (decreasing total p38 and BiP and increasing p-JNK2/total JNK2 ratio, and caspase 3) and gene expressions (decreasing BiP and increasing IRE1 and PERK) of ERS and autophagy (decreasing Beclin and increasing LC3 and ATG14) markers in soleus. Both FOs attenuated (p < 0.05) the increase in PERK and ATG14 expressions induced by HS. Thus, both FOs could potentially attenuate ERS and autophagy in skeletal muscles undergoing atrophy. |
| Databáze: | OpenAIRE |
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