Structural insight into microtubule stabilization and kinesin inhibition by Tau family MAPs
| Autor: | Ryo Nitta, Takuya Sumi, Mikako Shirouzu, Chihiro Doki, Mari Aoki, Kiyotaka Tokuraku, Ayako Sakamoto, Hideki Shigematsu, Tomomi Uchikubo-Kamo, Tsuyoshi Imasaki |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Alternative splicing Motility Kinesins Cell Biology macromolecular substances Biology Microtubule stabilization Microtubules Article 03 medical and health sciences 030104 developmental biology Tubulin Microtubule biology.protein Biophysics Molecular motor Kinesin Humans Microtubule-Associated Proteins Physiological Phenomenon Research Articles |
| Zdroj: | The Journal of Cell Biology |
| ISSN: | 1540-8140 |
| Popis: | The Tau family of microtubule-associated proteins promote microtubule stabilization or regulate microtubule-based motility. Shigematsu et al. visualized MAP4 and microtubules complexed with kinesin-1 by cryo-EM, which suggests a structural basis of microtubule stabilization and kinesin inhibition by Tau family MAPs. The Tau family microtubule-associated proteins (MAPs) promote microtubule stabilization and regulate microtubule-based motility. They share the C-terminal microtubule-binding domain, which includes three to five tubulin-binding repeats. Different numbers of repeats formed by alternative splicing have distinct effects on the activities of these proteins, and the distribution of these variants regulates fundamental physiological phenomena in cells. In this study, using cryo-EM, we visualized the MAP4 microtubule complex with the molecular motor kinesin-1. MAP4 bound to the C-terminal domains of tubulins along the protofilaments stabilizes the longitudinal contacts of the microtubule. The strongest bond of MAP4 was found around the intertubulin–dimer interface such that MAP4 coexists on the microtubule with kinesin-1 bound to the intratubulin–dimer interface as well. MAP4, consisting of five repeats, further folds and accumulates above the intertubulin–dimer interface, interfering with kinesin-1 movement. Therefore, these cryo-EM studies reveal new insight into the structural basis of microtubule stabilization and inhibition of kinesin motility by the Tau family MAPs. Graphical Abstract |
| Databáze: | OpenAIRE |
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