Gingival crevicular fluid can degrade Emdogain and inhibit Emdogain-induced proliferation of periodontal ligament fibroblasts

Autor: Timo Sorsa, James P. Simmer, S. Vardar-Sengul, Gül Atilla, Haluk Baylas, T Salo, B. Han Saygan, Matti Laaksonen
Přispěvatelé: Ege Üniversitesi
Jazyk: angličtina
Rok vydání: 2010
Předmět:
Adult
Male
Emdogain
matrix metalloproteinase
Adolescent
Periodontal Ligament
proliferation
Alveolar Bone Loss
Cell Culture Techniques
Connective tissue
Dentistry
Matrix metalloproteinase
Andrology
Young Adult
Dental Enamel Proteins
Matrix Metalloproteinase 13
Periodontal Attachment Loss
Matrix Metalloproteinase 14
medicine
Humans
Periodontal Pocket
Periodontal fiber
Zymography
Fibroblast
periodontitis
Periodontal Diseases
Cell Proliferation
Periodontitis
business.industry
Fibroblasts
Middle Aged
medicine.disease
Gingivitis
amelogenin
Matrix Metalloproteinase 8
medicine.anatomical_structure
Aggressive Periodontitis
Matrix Metalloproteinase 9
gingival crevicular fluid
Chronic Periodontitis
Ligament
Matrix Metalloproteinase 2
Periodontics
Female
Matrix Metalloproteinase 1
Amelogenin
Gingival Hemorrhage
business
ISSN: 0002-7669
Popis: WOS: 000276699300008
PubMed ID: 19909398
Background and Objective: Emdogain (R) (EMD), consisting mostly of amelogenin, is used in periodontal therapy to regenerate lost connective tissue. Emdogain is applied onto periodontally affected root surfaces, where it becomes exposed to proteolytic enzymes. In this study, we aimed to find out whether gingival crevicular fluid or matrix metalloproteinases (MMPs) could degrade EMD, and whether this degradation has consequences for in vitro cell proliferation. Material and Methods: We studied the effects of 156 gingival crevicular fluid samples collected from subjects with different stages of periodontal disease and from healthy control subjects and the effects of MMP-1, -2, -8, -9, -13 and -14 on the degradation of EMD using EMD-embedded zymography. The effects of gingival crevicular fluid with or without EMD and the effects of amelogenin on the proliferation of cultured periodontal ligament fibroblasts were studied by cell proliferation enzyme-linked immunosorbent assay kit. Results: Degradation of Emdogain induced by gingival crevicular fluid was greater in samples from all stages of periodontal diseases compared with healthy control samples. Of the MMPs studied, only MMP-2 and MMP-8 showed limited EMD-degrading activities. One hundred micrograms per millilitre of EMD increased proliferation of periodontal ligament fibroblasts on average by 24% (confidence interval 0.60-0.64) and at 200 mu g/mL by 30% (confidence interval 0.62-0.68) compared with control fibroblasts (confidence interval 0.48-0.52). However, gingival crevicular fluid (10 mu g/mL) together with 100 mu g/mL EMD induced the proliferation only by 6% (confidence interval 0.51-0.55) and with 200 mu g/mL EMD by 12% (confidence interval 0.54-0.58). Amelogenin at 200 mu g/mL decreased the proliferation of periodontal ligament fibroblasts by 54% (confidence interval 0.22-0.25). Conclusion: We suggest that diseased gingival crevicular fluid containing various proteases leads to degradation of EMD and decreased proliferation of periodontal ligament fibroblasts.
Academy of FinlandAcademy of Finland; Helsinki University Central Hospital Research Foundation; Oulu University Central Hospital Research Foundation; Finnish Dental Society Apollonia
The skilful technical assistance of Ms Maija-Leena Lehtonen (at the University of Oulu) is greatly appreciated. This study was supported by grants from the Academy of Finland, the Helsinki University Central Hospital Research Foundation, the Oulu University Central Hospital Research Foundation and the Finnish Dental Society Apollonia. We ensure that none of the authors have any financial or personal relationships with other people or organizations that could inappropriately influence our work.
Databáze: OpenAIRE
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