Blockade of IL-33/ST2 ameliorates airway inflammation in a murine model of allergic asthma

Autor:	Ji Young Kang, Soon Suk Kwon, Ji Hae Byun, Seung Joon Kim, Chin Kook Rhee, Hea Yon Lee, Young Kyoon Kim, Sook Young Lee, Joon Young Choi
Rok vydání:	2014
Předmět:	Pulmonary and Respiratory Medicine Allergy Ovalbumin Clinical Biochemistry Interleukin-1 Receptor-Like 1 Protein Inflammation Mice Th2 Cells medicine Animals Molecular Biology Mice Inbred BALB C biology medicine.diagnostic_test business.industry Interleukins Interleukin Pneumonia Receptors Interleukin respiratory system Eosinophil Immunoglobulin E medicine.disease Interleukin-33 Asthma respiratory tract diseases Antibodies Anti-Idiotypic Interleukin 33 Disease Models Animal medicine.anatomical_structure Bronchoalveolar lavage Immunoglobulin G Immunology biology.protein Cytokines Female medicine.symptom business Bronchoalveolar Lavage Fluid Signal Transduction
Zdroj:	Experimental lung research. 40(2)
ISSN:	1521-0499
Popis:	Interleukin (IL)-33 is involved in the development of lung inflammation by inducing or amplifying Th2 type-mediated responses in various animal models of allergic asthma. The ST2 gene is a member of the IL-1 receptor family, producing a transmembrane form (ST2L) and a soluble secreted form (sST2). sST2 has been shown to block this IL-33/ST2 signaling pathway. This study aimed to investigate whether anti-IL-33 and sST2 reduced airway inflammation in a murine model of asthma.BALB/c mice were sensitized and challenged with ovalbumin (OVA), and the effect of sST2 and anti-IL-33 antibody on airway inflammation and airway hyperresponsiveness (AHR) was evaluated. Furthermore, we measured changes in various cytokines in the bronchoalveolar lavage (BAL) fluid when treated with sST2 or anti-IL-33.We observed that anti-IL-33 antibody and sST2 exert a negative regulation on OVA-mediated allergic airway inflammation. Both treatments reduced total cell counts and eosinophil counts in BAL fluid and AHR to methacholine. The Th2 cytokines, such as IL-4, IL-5, and IL-13 in BAL fluid were also significantly decreased after both treatments. However, there were no changes in the level of TGF- ß1 and IL-10 after each treatment.These results suggest that anti-IL-33 as well as sST2 have therapeutic potential for allergic asthma through inhibition of Th2 cytokine production.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::44cbdda7d9f56e12c7d5ab64186683f0 https://pubmed.ncbi.nlm.nih.gov/24446582 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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