Large-scale identification of differentially expressed genes during neurogenesis
| Autor: | Cecilia Pazman, Joann C. Castelli, Xiling Wen, Roland Somogyi |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Central Nervous System
Neurons Genetics Messenger RNA Reverse Transcriptase Polymerase Chain Reaction General Neuroscience Molecular Sequence Data Neurogenesis Gene Expression Regulation Developmental Biology Embryo Mammalian PC12 Cells Embryonic stem cell Rats Cell biology Real-time polymerase chain reaction Nerve growth factor Spinal Cord Cell culture Gene expression Animals RNA Messenger Gene |
| Zdroj: | NeuroReport. 11:719-724 |
| ISSN: | 0959-4965 |
| Popis: | We report here a modified mRNA differential display method and its application for the analysis of differential gene expression in NGF-treated PC12 cells and in embryonic rat spinal cord. The optimized protocol is based on low fidelity priming of multiple cDNAs followed by high fidelity amplification. In PC12 cells induction by nerve growth factor (NGF) altered the expression of 4% of the 466 transcripts evaluated. During neurogenesis of the spinal cord we found that 30% of the 288 examined products changed. The differential expression of the characterized genes was confirmed by independent quantitative PCR. We conclude this method is suitable for the identification of increases and decreases of mRNA levels and allows the discovery of differentially expressed unknown transcripts. |
| Databáze: | OpenAIRE |
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