Rice Black-streaked Dwarf Virus Preparation and Infection on Rice
| Autor: | Kaili Xie, Hengmu Zhang, Zhang Hehong, Sun Zongtao, Chen Jianping, Xiaoxiang Tan |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
education.field_of_study
biology Inoculation Strategy and Management Mechanical Engineering Population Metals and Alloys food and beverages Genus Fijivirus biology.organism_classification Industrial and Manufacturing Engineering Virus Planthopper Rice black-streaked dwarf virus Agronomy Methods Article Brown planthopper Functional studies education |
| Zdroj: | Bio Protoc |
| ISSN: | 2331-8325 |
| Popis: | Rice black-streaked dwarf virus (RBSDV), a member of genus Fijivirus in the family Reoviridae, infects rice, maize, barley and wheat, and can seriously affect crop yields. RBSDV is transmitted by the small brown planthopper (Laodelphax striatellus, SBPH) in a persistent manner. RBSDV has 10 linear dsRNA genomic segments, making it difficult to construct infectious clones for functional studies in plants. Here we describe a method for inoculating and maintaining RBSDV on rice in a greenhouse for use in laboratory research. The protocol uses SBPHs mass reared in the laboratory. We also describe in detail the propagation of a healthy planthopper population, the preparation of plant material, RBSDV inoculation and the evaluation of the rice after inoculation. |
| Databáze: | OpenAIRE |
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