Transcript Abundance of Putative Lipid Phosphate Phosphatases during Development of Trypanosoma brucei in the Tsetse Fly
| Autor: | Serap Aksoy, Amy F. Savage, Thiago Luiz Alves e Silva |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Tsetse Flies Cellular differentiation 030231 tropical medicine Trypanosoma brucei brucei Phosphatidate Phosphatase Gene Expression Trypanosoma brucei Genome 03 medical and health sciences 0302 clinical medicine Virology Gene expression parasitic diseases Parasite hosting Animals Gene Genetics biology Gene Expression Profiling Tsetse fly Articles biology.organism_classification Molecular biology Gene expression profiling 030104 developmental biology Infectious Diseases Parasitology |
| Popis: | African trypanosomes (Trypanosoma brucei spp.) cause devastating diseases in sub-Saharan Africa. Trypanosomes differentiate repeatedly during development in tsetse flies before gaining mammalian infectivity in fly salivary glands. Lipid phosphate phosphatases (LPPs) are involved in diverse biological processes, such as cell differentiation and cell migration. Gene sequences encoding two putative T. brucei LPP proteins were used to search the T. brucei genome, revealing two additional putative family members. Putative structural features and transcript abundance during parasite development in tsetse fly were characterized. Three of the four LPP proteins are predicted to have six transmembrane domains, while the fourth shows only one. Semiquantitative gene expression revealed differential regulation of LPPs during parasite development. Transcript abundance for three of the four putative LPP genes was elevated in parasites infecting salivary glands, but not mammalian-infective metacyclic cells in fly saliva, indicating a potential role of this family in parasite establishment in tsetse salivary glands. |
| Databáze: | OpenAIRE |
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