Short-term administration of spexin in rats reduces obesity by affecting lipolysis and lipogenesis: An in vivo and in vitro study
| Autor: | Dawid Szczepankiewicz, Paweł Kołodziejski, Maciej Sassek, Krzysztof W. Nowak, Ewa Pruszyńska-Oszmałek |
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| Rok vydání: | 2020 |
| Předmět: |
Male
medicine.medical_specialty Lipolysis Peptide Hormones Adipose tissue 030209 endocrinology & metabolism Biology In Vitro Techniques 03 medical and health sciences 0302 clinical medicine Endocrinology Internal medicine medicine Adipocytes Animals Insulin Obesity Phosphorylation Rats Wistar Receptor 030304 developmental biology 0303 health sciences Leptin Lipogenesis Lipids Rats Glucose Perilipin Animal Science and Zoology Insulin Resistance Galanin receptor 3 Galanin receptor 2 |
| Zdroj: | General and comparative endocrinology. 299 |
| ISSN: | 1095-6840 |
| Popis: | The present study aimed to characterize the role of spexin (SPX) in maintaining glucose and lipid homeostasis in vivo in rats with diet-induced obesity. The in vitro effect of spexin on metabolic and endocrine functions of adipocytes isolated from obese rats was also investigated. The in vivo experiment was conducted on rats with diet-induced obesity and administered with SPX for 7 days. Lipid and carbohydrate parameters, liver markers, and hormonal profile were measured. In in vitro studies, adipocytes isolated from obese rats were used. The effect of SPX on lipolysis, lipogenesis, and leptin secretion from fat cells was assessed. The results showed that short-term administration of SPX causes weight loss, increases insulin sensitivity, and improves the metabolic state of obese rats. The in vitro experiments showed that spexin and its receptors, namely galanin receptor 2 (GALR2) and galanin receptor 3 (GALR3), were expressed in various fat depots and in adipocytes from obese rats. We also found that the addition of spexin increased the basal and isoproterenol-stimulated lipolysis and reduced the basal and insulin-stimulated lipogenesis in adipocytes isolated from obese rats. Molecular analysis showed that SPX activated hormone-sensitive lipase (HSL) phosphorylation and upregulated perilipin and HSL mRNA expression. These results suggest that SPX regulates metabolism of obese rats by affecting lipolysis and lipogenesis in adipocytes. Moreover, the present study for the first time demonstrates that SPX modulates leptin synthesis and secretion from isolated adipocytes. |
| Databáze: | OpenAIRE |
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