Vaccination with recombinant vaccinia viruses expressing ICP27 induces protective immunity against herpes simplex virus through CD4+ Th1+ T cells
| Autor: | D. Gheysen, Moncef Slaoui, Nelly A. Kuklin, Chantal Molitor, Elanchezhiyan Manickan, M. Dewerchin, M. Francotte, Barry T. Rouse |
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| Rok vydání: | 1995 |
| Předmět: |
medicine.medical_treatment
viruses Herpesvirus 2 Human Immunology Vaccinia virus Mice SCID Biology medicine.disease_cause Microbiology Immediate early protein law.invention Immediate-Early Proteins Mice law Immunity Virology medicine Animals Immunity Cellular Mice Inbred BALB C Mice Inbred C3H Vaccines Synthetic Viral Vaccines Th1 Cells In vitro Herpes simplex virus Cytokine Immunization Insect Science Recombinant DNA Female CD8 Research Article |
| Zdroj: | Journal of virology. 69(8) |
| ISSN: | 0022-538X |
| Popis: | This study was designed to evaluate the efficacy and mechanisms of protection mediated by recombinant vaccinia viruses encoding immediate-early (IE) proteins of herpes simplex virus type 2 (HSV-2). Three mouse strains were immunized against the IE proteins ICP27, ICP0, and ICP4, and mice were challenged intracutaneously in the zosteriform model with HSV-2 strain MS. Protection was observed only following immunization with the ICP27 construct and then only in the BALB/c mouse strain. Protection in BALB/c mice was ablated by CD4+ T-cell suppression but remained intact in animals depleted of CD8+ T cells. Moreover, protection could be afforded to SCID nude recipients with CD4+ but not CD8+ T cells from ICP27-immunized mice. Only BALB/c mice developed a delayed-type hypersensitivity reaction to HSV-2, and in vitro measurements of humoral and cell-mediated immunity revealed response patterns to ICP27 and HSV that differed between protected BALB/c and unprotected mouse strains. Accordingly, BALB/c responses showed antigen-induced cytokine profiles dominated by type 1 cytokines, whereas C57BL/6 and C3H/HeN mice generated cytokine responses mainly of the type 2 variety. Our results may indicate that protection against zosterification is mainly mediated by CD4+ T cells that express a type 1 cytokine profile and that protective vaccines against HSV which effectively induce such T-cell responses should be chosen. |
| Databáze: | OpenAIRE |
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