Effects of tumor necrosis factor on viral replication and pulmonary inflammation during acute mouse adenovirus type 1 respiratory infection
| Autor: | Krittika Pant, Adithya Chandrasekaran, Jason B. Weinberg, Christine Chang, Aditya Vageesh, Alexandra J. Popkov |
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| Rok vydání: | 2020 |
| Předmět: |
Male
Adenoviridae Infections Viral pathogenesis CD8-Positive T-Lymphocytes Biology Virus Replication Article Proinflammatory cytokine Mastadenovirus Mice 03 medical and health sciences Immune system Virology Animals Humans Cytotoxic T cell Lung 030304 developmental biology 0303 health sciences Tumor Necrosis Factor-alpha 030302 biochemistry & molecular biology Respiratory infection Pneumonia Interleukin-10 Mice Inbred C57BL Viral replication Immunology Tumor necrosis factor alpha Viral load |
| Zdroj: | Virology |
| ISSN: | 0042-6822 |
| DOI: | 10.1016/j.virol.2020.05.004 |
| Popis: | CD8 T cells contribute to effective clearance of mouse adenovirus type 1 (MAV-1) and to virus-induced pulmonary inflammation. We characterized effects of a CD8 T cell effector, TNF, on MAV-1 pathogenesis. TNF inhibited MAV-1 replication in vitro. TNF deficiency or immunoneutralization had no effect on lung viral loads or viral gene expression in mice infected intranasally with MAV-1. Absence of TNF delayed virus-induced weight loss and reduced histological evidence of pulmonary inflammation, although concentrations of proinflammatory cytokines and chemokines in bronchoalveolar lavage fluid (BALF) were not significantly affected. BALF concentrations of IL-10 were greater in TNF-deficient mice compared to controls. Our data indicate that TNF is not essential for control of viral replication in vivo, but virus-induced TNF contributes to some aspects of immunopathology and disease. Redundant CD8 T cell effectors and other aspects of immune function are sufficient for antiviral and pro-inflammatory responses to acute MAV-1 respiratory infection. |
| Databáze: | OpenAIRE |
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