Effect of zymosan and poly (I:C) adjuvants on responses to microneedle immunization coated with whole inactivated influenza vaccine
| Autor: | Jin-Yong Noh, Daeyoon Jung, Seong Dong Jeong, Chang-Seon Song, Jae-Keun Park, Ju-Hyung Shin, Kwon-Ho Kim, Yeu-Chun Kim, Ji-Ho Lee |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Microinjections medicine.drug_class Influenza vaccine medicine.medical_treatment Pharmaceutical Science 02 engineering and technology Administration Cutaneous Immunostimulant 03 medical and health sciences chemistry.chemical_compound Drug Delivery Systems Influenza A Virus H1N1 Subtype Adjuvants Immunologic Orthomyxoviridae Infections Antigen medicine Animals Humans Adjuvants Pharmaceutic Immunity Cellular Mice Inbred BALB C business.industry Immunogenicity Vaccination Zymosan 021001 nanoscience & nanotechnology Vaccine efficacy Virology Poly I-C 030104 developmental biology Vaccines Inactivated chemistry Influenza Vaccines Needles Immunology Female 0210 nano-technology business Adjuvant |
| Zdroj: | Journal of Controlled Release. 265:83-92 |
| ISSN: | 0168-3659 |
| DOI: | 10.1016/j.jconrel.2017.09.010 |
| Popis: | Microneedles are the micrometer size devices used for the delivery of vaccines and biotherapeutics. In order to increase the vaccine efficacy and reduce the antigen dose, there is a significant need to find some adjuvants for the microneedle vaccination. In this study, zymosan, which is the cell wall preparation of Saccharomyces cerevisiae, or poly (I:C) was coated on a microneedle with inactivated influenza virus, and then immunized into BALB/c mouse to determine the immunogenicity, protection and synergetic effect between two adjuvants. As a result, the group administered with zymosan and vaccine antigen showed significantly stronger IgG response, HI titer and IgG subtypes without any adverse effects, compared to the group immunized with the vaccine antigen alone. Also, there were enhanced cellular immune responses in the group received adjuvant with vaccine antigen. In addition, they showed superior protection and lung viral reduction against lethal viral challenge. Taken together, this study confirms that zymosan can be used as an immunostimulant for microneedle vaccination. |
| Databáze: | OpenAIRE |
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