Blueberry (Vaccinium virgatum) Leaf Extracts Protect Against Aβ-Induced Cytotoxicity and Cognitive Impairment
| Autor: | Ho Jin Heo, Yu Na Jo, Ji Hee Jeong, Hee Rok Jeong, Hyeon Ju Kim, Man-Jo Kim |
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| Rok vydání: | 2013 |
| Předmět: |
Male
Antioxidant Cell Survival medicine.medical_treatment Blueberry Plants Medicine (miscellaneous) medicine.disease_cause PC12 Cells Antioxidants Mice chemistry.chemical_compound Cognition Phenols In vivo Malondialdehyde Lactate dehydrogenase medicine Animals Neurons chemistry.chemical_classification Memory Disorders Mice Inbred ICR Reactive oxygen species Amyloid beta-Peptides Nutrition and Dietetics Dose-Response Relationship Drug L-Lactate Dehydrogenase biology Plant Extracts Vaccinium virgatum biology.organism_classification Rats Plant Leaves Oxidative Stress chemistry Biochemistry Cholinesterase Inhibitors Chlorogenic Acid Cognition Disorders Reactive Oxygen Species Oxidative stress Phytotherapy |
| Zdroj: | Journal of Medicinal Food. 16:968-976 |
| ISSN: | 1557-7600 1096-620X |
| Popis: | The ethylacetate (EtOAc) fraction of blueberry leaf extract was investigated to examine the in vivo antiamnesic effects against amyloid β protein (Aβ)-induced learning and memory deficit. The fraction showed the highest antioxidant activities, and the generation of intracellular reactive oxygen species was significantly decreased. Cell viability assays revealed the in vitro cytoprotective effects of the fraction, and the cytoplasmic lactate dehydrogenase release into the medium was dose-dependently inhibited. In addition, a chlorogenic acid was identified as a predominant phenolic compound by high-performance liquid chromatography analysis. Antiamnesic effects were evaluated by using in vivo the Y-maze and passive avoidance tests, and preadministration of the fraction attenuated Aβ-induced memory impairment in both in vivo experiments. Acetylcholinesterase prepared from mice brain was inhibited by the fraction, and malondialdehyde generation in the brain homogenate was also decreased. These findings suggest that the EtOAc fraction of blueberry leaf extract could possess a wide range of physiological effects against neurodegenerative diseases. |
| Databáze: | OpenAIRE |
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