l-Arginine supplementation induces glutathione synthesis in interscapular brown adipose tissue through activation of glutamate-cysteine ligase expression: The role of nitric oxide
| Autor: | Ana Vasilijević, Aleksandra Jankovic, Bato Korac, V.M. Petrović, Biljana Buzadžić, Aleksandra Korac |
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| Rok vydání: | 2009 |
| Předmět: |
Male
Arginine Nitric Oxide Synthase Type III Glutamate-Cysteine Ligase Adipose tissue Gene Expression Nitric Oxide Synthase Type II Toxicology Nitric Oxide Nitric oxide 03 medical and health sciences chemistry.chemical_compound Adipose Tissue Brown In vivo Brown adipose tissue medicine Animals RNA Messenger Enzyme Inhibitors 030304 developmental biology chemistry.chemical_classification 0303 health sciences DNA ligase 030302 biochemistry & molecular biology NF-κB General Medicine Glutathione Molecular biology Rats medicine.anatomical_structure NG-Nitroarginine Methyl Ester chemistry |
| Zdroj: | Chemico-biological interactions. 182(2-3) |
| ISSN: | 1872-7786 |
| Popis: | We examined whether nitric oxide (NO) in vivo could induce interscapular brown adipose tissue (IBAT) glutathione synthesis. Data show that NO induces in vivo IBAT glutathione synthesis through activation of glutamate-cysteine ligase (GCL) mRNA and protein expression. This NO effect appeared to be mediated by nuclear factor-κB (NF-κB) activation. We have also observed a complex series of in vivo cellular responses during chronic inhibition of NO synthesis, suggesting that regulatory pathways unrelated to GCL alteration underlie glutathione level increase induced by Nω-nitro- l -arginine methyl ester ( l -NAME). In general, glutathione synthesis in IBAT seemed to be finely tuned by NO to provide glutathione for either mediating the effects of NO, or for preventing potential nitrosative stress. |
| Databáze: | OpenAIRE |
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