Fine Needle-Diathermy Regresses Pathological Corneal (Lymph)Angiogenesis and Promotes High-Risk Corneal Transplant Survival
| Autor: | Ann-Charlott Schneider, Viet Nhat Hung Le, Felix Bock, Claus Cursiefen, Rebecca Scholz |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Graft Rejection
0301 basic medicine CD31 Pathology medicine.medical_specialty Angiogenesis medicine.medical_treatment Anti-Inflammatory Agents lcsh:Medicine Article Corneal Transplantation Mice 03 medical and health sciences 0302 clinical medicine Diathermy Animals Medicine Corneal Neovascularization Lymphangiogenesis lcsh:Science Glycoproteins Mice Inbred BALB C Multidisciplinary business.industry Graft Survival lcsh:R Membrane Transport Proteins Corneal Transplant medicine.disease 3. Good health Platelet Endothelial Cell Adhesion Molecule-1 Disease Models Animal Treatment Outcome 030104 developmental biology Lymphatic system Needles Corneal neovascularization 030221 ophthalmology & optometry Cauterization Female lcsh:Q Lymph business |
| Zdroj: | Scientific Reports Scientific Reports, Vol 8, Iss 1, Pp 1-8 (2018) |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-018-24037-3 |
| Popis: | Pathological corneal hem- and lymphangiogenesis are prime risk factors for corneal graft rejection. Fine needle-diathermy (FND) is an option to regress corneal blood vessels; however, whether this treatment besides clinically visible blood vessels also affects invisible lymphatic vessels is so far unknown. Here we test the hypothesis that FND destroys not only blood but also lymphatic vessels, thereby promotes corneal high-risk graft survival. The effect of FND was studied in vivo using BALB/c mice and the model of suture-induced corneal neovascularization. Mice were divided into three groups: FND, ANTI (anti-inflammatory therapy) and NON (control). Five, 7, 10 and 20 days after cauterization, corneas were harvested and stained with LYVE-1, CD31 to quantify (lymph)angiogenesis. The long-term survival of allografts was compared between the three groups. FND caused significant regression of both blood and lymphatic vessels compared to the control group at all time points (p |
| Databáze: | OpenAIRE |
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