Contrasting roles of macrophages and dendritic cells in controlling initial pulmonary Brucella infection

Autor: Béatrice de Bovis, Hugues Lelouard, Nico van Rooijen, Bernard Malissen, Cristel Archambaud, Jean-Pierre Gorvel, Elisabeth Devilard, Suzana P. Salcedo
Přispěvatelé: Centre d'Immunologie de Marseille - Luminy (CIML), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU), Technologies avancées pour le génôme et la clinique (TAGC), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Department of Molecular Cell Biology [Amsterdam UMC], Vrije Universiteit Medical Centre (VUMC), Vrije Universiteit Amsterdam [Amsterdam] (VU)-Vrije Universiteit Amsterdam [Amsterdam] (VU), CNRS, INSERM, ANR, FRM
Jazyk: angličtina
Rok vydání: 2010
Předmět:
MESH: Macrophages
Alveolar
Brucella abortus
Nitric Oxide Synthase Type II
Cell Count
pulmonary infection
MESH: Lymph Nodes
MESH: Bacterial Load
DC
Mice
0302 clinical medicine
Cell Movement
Immunology and Allergy
MESH: Animals
Lung
MESH: Brucella abortus
MESH: Cell Movement
0303 health sciences
medicine.diagnostic_test
MESH: Dendritic Cells
respiratory system
3. Good health
macrophages
medicine.anatomical_structure
nasal infection
[SDV.IMM]Life Sciences [q-bio]/Immunology
Tumor necrosis factor alpha
MESH: Nitric Oxide Synthase Type II
MESH: Immunity
Innate
Immunology
Brucella
Biology
Brucellosis
Flow cytometry
03 medical and health sciences
Immune system
Immunity
MESH: Mice
Inbred C57BL
MESH: Brucellosis
Parenchyma
Macrophages
Alveolar
medicine
Animals
MESH: Lung
MESH: Mice
030304 developmental biology
Innate immune system
Tumor Necrosis Factor-alpha
MESH: Cell Count
Dendritic Cells
biology.organism_classification
Bacterial Load
Immunity
Innate
Mice
Inbred C57BL
MESH: Tumor Necrosis Factor-alpha
Lymph Nodes
030215 immunology
Zdroj: European Journal of Immunology
European Journal of Immunology, Wiley-VCH Verlag, 2010, 40 (12), pp.3458-71. ⟨10.1002/eji.201040497⟩
European Journal of Immunology, 2010, 40 (12), pp.3458-71. ⟨10.1002/eji.201040497⟩
ISSN: 0014-2980
1521-4141
DOI: 10.1002/eji.201040497⟩
Popis: International audience; Control of pulmonary pathogens constitutes a challenging task as successful immune responses need to be mounted without damaging the lung parenchyma. Using immunofluorescence microscopy and flow cytometry, we analyzed in the mouse the initial innate immune response that follows intranasal inoculation of Brucella abortus. Bacteria were absent from parenchymal dendritic cells (DC) but present in alveolar macrophages in which they replicated. When the number of alveolar macrophages was reduced prior to Brucella infection, small numbers of pulmonary DC were infected and a massive recruitment of TNF-α- and iNOS-producing DC ensued. Coincidentally, Brucella disseminated to the lung-draining mediastinal lymph nodes (LN) where they replicated in both migratory DC and migratory alveolar macrophages. Together, these results demonstrate that alveolar macrophages are critical regulators of the initial innate immune response against Brucella within the lungs and show that pulmonary DC and alveolar macrophages play rather distinct roles in the control of microbial burden.
Databáze: OpenAIRE
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