Pro-atherogenic effect of interleukin-4 in endothelial cells: Modulation of oxidative stress, nitric oxide and monocyte chemoattractant protein-1 expression

Autor: David-Dufilho, Monique, Walch, L., Massade, L., Dufilho, M., Brunet, A., Rendu, F.
Přispěvatelé: Unité de Recherche sur les Maladies Cardiovasculaires, du Métabolisme et de la Nutrition = Institute of cardiometabolism and nutrition (ICAN), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)
Jazyk: angličtina
Rok vydání: 2006
Předmět:
Umbilical Veins
Morpholines
Nitric Oxide Synthase Type II
Arachidonic Acids
030204 cardiovascular system & hematology
medicine.disease_cause
Nitric Oxide
Nitric oxide
Superoxide dismutase
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
medicine
Humans
[SDV.BBM]Life Sciences [q-bio]/Biochemistry
Molecular Biology

RNA
Messenger

Enzyme Inhibitors
Chemokine CCL2
ComputingMilieux_MISCELLANEOUS
030304 developmental biology
chemistry.chemical_classification
0303 health sciences
Reactive oxygen species
NADPH oxidase
biology
Reverse Transcriptase Polymerase Chain Reaction
Tumor Necrosis Factor-alpha
Monocyte
NADPH Oxidases
Atherosclerosis
Molecular biology
Endothelial stem cell
Oxidative Stress
medicine.anatomical_structure
Biochemistry
chemistry
NAD(P)H oxidase
Chromones
biology.protein
Endothelium
Vascular

Interleukin-4
Cardiology and Cardiovascular Medicine
Reactive Oxygen Species
Oxidative stress
Zdroj: Atherosclerosis
Atherosclerosis, Elsevier, 2006, 187 (2), pp.285-291. ⟨10.1016/j.atherosclerosis.2005.09.016⟩
ISSN: 0021-9150
DOI: 10.1016/j.atherosclerosis.2005.09.016⟩
Popis: Background Although considered as an anti-inflammatory cytokine, interleukin-4 (IL-4) has been shown to be pro-atherogenic in mice models of atherosclerosis. Objectives In order to elucidate this paradox, we have investigated the effects of IL-4 on characteristic atherogenic parameters in human umbilical vein endothelial cells (HUVECs): production of reactive oxygen species, expression of monocyte chemoattractant protein-1 (MCP-1) and nitric oxide (NO) bioavailability. Results Incubation of HUVECs with IL-4 resulted in an increased production of reactive oxygen species and extracellular O 2 − measured using fluorogenic probes and Cytochrome c that was inhibited by superoxide dismutase or gp91ds- tat , a selective NADPH oxidase inhibitor. The latter also inhibited IL-4 induced over-expression of MCP-1 mRNA measured by classical and real time RT-PCR. Incubation of HUVECs with IL-4 reduced thrombin-induced NO release, detected by electrochemistry, an effect which was reversed by incubation with superoxide dismutase. Both production of reactive oxygen species and MCP-1 mRNA over-expression induced by IL-4 were fully inhibited by selective inhibitors of phosphatidyl inositol 3-kinase. Conclusion The data demonstrate that IL-4 up-regulates the expression of MCP-1 and decreases NO bioavailability through activation of NADPH oxidase in endothelial cells. These results are in favor of a pro-inflammatory and pro-atherogenic effect of IL-4 in vascular tissues.
Databáze: OpenAIRE