Pro-atherogenic effect of interleukin-4 in endothelial cells: Modulation of oxidative stress, nitric oxide and monocyte chemoattractant protein-1 expression
Autor: | David-Dufilho, Monique, Walch, L., Massade, L., Dufilho, M., Brunet, A., Rendu, F. |
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Přispěvatelé: | Unité de Recherche sur les Maladies Cardiovasculaires, du Métabolisme et de la Nutrition = Institute of cardiometabolism and nutrition (ICAN), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU) |
Jazyk: | angličtina |
Rok vydání: | 2006 |
Předmět: |
Umbilical Veins
Morpholines Nitric Oxide Synthase Type II Arachidonic Acids 030204 cardiovascular system & hematology medicine.disease_cause Nitric Oxide Nitric oxide Superoxide dismutase 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine Humans [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology RNA Messenger Enzyme Inhibitors Chemokine CCL2 ComputingMilieux_MISCELLANEOUS 030304 developmental biology chemistry.chemical_classification 0303 health sciences Reactive oxygen species NADPH oxidase biology Reverse Transcriptase Polymerase Chain Reaction Tumor Necrosis Factor-alpha Monocyte NADPH Oxidases Atherosclerosis Molecular biology Endothelial stem cell Oxidative Stress medicine.anatomical_structure Biochemistry chemistry NAD(P)H oxidase Chromones biology.protein Endothelium Vascular Interleukin-4 Cardiology and Cardiovascular Medicine Reactive Oxygen Species Oxidative stress |
Zdroj: | Atherosclerosis Atherosclerosis, Elsevier, 2006, 187 (2), pp.285-291. ⟨10.1016/j.atherosclerosis.2005.09.016⟩ |
ISSN: | 0021-9150 |
DOI: | 10.1016/j.atherosclerosis.2005.09.016⟩ |
Popis: | Background Although considered as an anti-inflammatory cytokine, interleukin-4 (IL-4) has been shown to be pro-atherogenic in mice models of atherosclerosis. Objectives In order to elucidate this paradox, we have investigated the effects of IL-4 on characteristic atherogenic parameters in human umbilical vein endothelial cells (HUVECs): production of reactive oxygen species, expression of monocyte chemoattractant protein-1 (MCP-1) and nitric oxide (NO) bioavailability. Results Incubation of HUVECs with IL-4 resulted in an increased production of reactive oxygen species and extracellular O 2 − measured using fluorogenic probes and Cytochrome c that was inhibited by superoxide dismutase or gp91ds- tat , a selective NADPH oxidase inhibitor. The latter also inhibited IL-4 induced over-expression of MCP-1 mRNA measured by classical and real time RT-PCR. Incubation of HUVECs with IL-4 reduced thrombin-induced NO release, detected by electrochemistry, an effect which was reversed by incubation with superoxide dismutase. Both production of reactive oxygen species and MCP-1 mRNA over-expression induced by IL-4 were fully inhibited by selective inhibitors of phosphatidyl inositol 3-kinase. Conclusion The data demonstrate that IL-4 up-regulates the expression of MCP-1 and decreases NO bioavailability through activation of NADPH oxidase in endothelial cells. These results are in favor of a pro-inflammatory and pro-atherogenic effect of IL-4 in vascular tissues. |
Databáze: | OpenAIRE |
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