Stress-Activated Protein Kinase Activation Is the Earliest Direct Correlate to the Induction of Secretagogue-Induced Pancreatitis in Rats
| Autor: | John A. Williams, Andrzej Dabrowski, Terrence Grady, Craig D. Logsdon |
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| Rok vydání: | 1996 |
| Předmět: |
Male
medicine.medical_specialty Trypsinogen Biophysics digestive system Biochemistry Cathepsin B chemistry.chemical_compound Internal medicine medicine Animals Rats Wistar Protein kinase A Molecular Biology Enzyme Precursors JNK Mitogen-Activated Protein Kinases Bombesin Colocalization Cell Biology medicine.disease Zymogen granule Rats Enzyme Activation Endocrinology Pancreatitis chemistry Calcium-Calmodulin-Dependent Protein Kinases Acute pancreatitis Receptors Cholecystokinin Secretagogue Mitogen-Activated Protein Kinases Ceruletide Subcellular Fractions |
| Zdroj: | Biochemical and Biophysical Research Communications. 227:1-7 |
| ISSN: | 0006-291X |
| DOI: | 10.1006/bbrc.1996.1458 |
| Popis: | We compared the cellular events induced by hyperstimulation of rats with caerulein which induces acute pancreatitis, to bombesin, which does not induce pancreatitis. Both secretogogues induced the intracellular activation of trypsinogen and the colocalization of lysosomal hydrolases and zymogen granules within 10–15 minutes. These data indicate that these parameters, previously thought to be crucial initiating events of pancreatitis, are not definitive cellular markers of the disease. We then compared the abilities of the two secretagogues to activate stress-activated protein kinase (SAPK). Significant effects of caerulein hyperstimulation on SAPK activity were observed within 5 minutes, the maximum (57-fold) activation was evident after 15 minutes, and levels remained above control for at least 3 hours. In comparison, hyperstimulation with bombesin induced a maximal 5-fold increase of SAPK activity which returned to basal within one hour. These data indicate that SAPK activity is the earliest and best correlated cellular marker associated with secretagogue-induced pancreatitis. |
| Databáze: | OpenAIRE |
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