Apoptosis of human gastric carcinoma cells induced byEuphorbia esulalatex

Autor: Xiao-Dong Han, Xiao-Bin Liu, Zhao-Ying Fu, Ai-Hong Wang
Rok vydání: 2016
Předmět:
inorganic chemicals
0301 basic medicine
Pathology
medicine.medical_specialty
Time Factors
Latex
Cell Survival
Cell
Apoptosis
Biology
Flow cytometry
03 medical and health sciences
0302 clinical medicine
Microscopy
Electron
Transmission
Downregulation and upregulation
Euphorbia
Stomach Neoplasms
Cell Line
Tumor
medicine
Fluorescence microscope
Humans
heterocyclic compounds
Viability assay
Cell Proliferation
Plants
Medicinal
Dose-Response Relationship
Drug
medicine.diagnostic_test
Plant Extracts
Reverse Transcriptase Polymerase Chain Reaction
Carcinoma
Gastroenterology
General Medicine
Basic Study
Flow Cytometry
Antineoplastic Agents
Phytogenic
Molecular biology
Staining
Gene Expression Regulation
Neoplastic
Reverse transcription polymerase chain reaction
030104 developmental biology
medicine.anatomical_structure
Microscopy
Fluorescence
030220 oncology & carcinogenesis
cardiovascular system
Apoptosis Regulatory Proteins
Phytotherapy
Signal Transduction
Zdroj: World Journal of Gastroenterology. 22:3564
ISSN: 1007-9327
Popis: AIM: To investigate the effect of Euphorbia esula (E. esula) extract in inhibiting proliferation and inducing apoptosis in SGC-7901 cells. METHODS: E. esula extract at different concentrations was used to inhibit proliferation and induce apoptosis of human gastric carcinoma SGC-7901 cells. Inhibition of proliferation was detected with thiazolyl blue assay, and apoptosis was detected with fluorescence microscopy, transmission electron microscopy, and flow cytometry. The mechanisms were studied by measurement of caspase-3 and caspase-8 activities and Bax and Bcl2 mRNA expression. RESULTS: The thiazolyl blue assay showed that SGC-7901 cell viability and proliferation were inhibited significantly by E. esula extract in a time- and concentration-dependent manner. Fluorescence microscopy revealed that the cell nuclei showed the characteristic changes of apoptosis, such as uneven staining and chromatin marginalization. Some key features of apoptosis were also observed under transmission electron microscopy, which included cellular shrinkage and the foaming or bubbling phenomenon. When the cells were analyzed by flow cytometry, a sub-G1 peak could be seen clearly. Spectrophotometric assay of caspase-3 and caspase-8 activities in the treated cells showed an approximately two-fold increase. Reverse transcription polymerase chain reaction showed that Bax mRNA expression was upregulated, while Bcl2 mRNA expression was downregulated. CONCLUSION: E. esula extract inhibited proliferation and induced apoptosis in SGC-7901 cells, in a caspase-dependent manner, involving upregulation of Bax and downregulation of Bcl2.
Databáze: OpenAIRE
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