Essential similarities between spontaneous and MeIQx-promoted aberrant crypt foci in the F344 rat colon
| ISSN: | 0304-3835 |
|---|---|
| Přístupová URL adresa: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::44ad0eb7e5a4bcbf388386cfe67b2d13 https://pubmed.ncbi.nlm.nih.gov/11566489 |
| Rights: | CLOSED |
| Přírůstkové číslo: | edsair.doi.dedup.....44ad0eb7e5a4bcbf388386cfe67b2d13 |
| Autor: | Akiyoshi Nishikawa, Zen-yo Tanakamaru, Fumio Furukawa, Hideki Mori, Ikuo Mori, Michihito Takahashi |
| Rok vydání: | 2001 |
| Předmět: |
Male
Cancer Research Pathology medicine.medical_specialty Dot blot medicine.disease_cause digestive system Proliferating Cell Nuclear Antigen Quinoxalines medicine Animals Carcinogen biology Cell growth Molecular biology digestive system diseases Rats Inbred F344 Proliferating cell nuclear antigen Rats Genes ras Oncology Toxicity Colonic Neoplasms Mutation biology.protein Carcinogens Immunohistochemistry Tumor Suppressor Protein p53 Carcinogenesis Precancerous Conditions Aberrant crypt foci Mutagens |
| Zdroj: | Cancer letters. 172(2) |
| ISSN: | 0304-3835 |
| Popis: | Aberrant crypt foci (ACFs) in the Fischer 344 (F344) rat colon, of control or 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MeIQx)-treated groups, were compared morphologically, immunohistochemically, and at the molecular biological level in order to elucidate their biological characteristics. Male 3-week-old rats were fed a diet supplemented with or without MeIQx at doses of 100 ppm or less for 16 weeks. The incidence of ACFs was the highest (90%) in animals given 100 ppm MeIQx but that in untreated rats was also surprisingly high (57%). Nine ACFs from nine MeIQx-treated rats and ten ACFs from ten untreated control rats were selected for detailed examination for their large size. There were no morphological differences in macroscopic and microscopic features between MeIQx-promoted and spontaneous ACFs. There were also no differences in immunohistochemical labeling for proliferating cell nuclear antigen (PCNA) and p53 protein between these ACFs although in both cases labeling was higher than in normal crypts. Dot blot hybridization revealed no c-K-ras mutations in codon 12 except in one ACF (11.1%) developing in a rat treated with 100 ppm MeIQx, in which a GGT-->GAT single base substitution was detected. Our results thus suggest that in terms of morphology, cell proliferation, P53 expression and c-K-ras mutation, most ACFs found in rats given 100 ppm MeIQx are essentially identical to their spontaneous counterparts. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect