Synergistic effect of microRNA and albumin-bound nanoparticles for inhibition of glioblastoma cancer cell proliferation
| Autor: | Rassoul Dinarvand, Masoud Soleimani, Maria Shariatnasery, Shiva Irani, Navid Goodarzi |
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| Rok vydání: | 2020 |
| Předmět: |
Cell cycle checkpoint
Paclitaxel Cell 030226 pharmacology & pharmacy 01 natural sciences Flow cytometry 03 medical and health sciences Pharmacy and materia medica 0302 clinical medicine Downregulation and upregulation Survivin medicine MTT assay Viability assay Combination therapy medicine.diagnostic_test Chemistry Albumin Cell cycle 0104 chemical sciences RS1-441 010404 medicinal & biomolecular chemistry medicine.anatomical_structure Cancer research Nanoparticles Glioblastoma miR-34a |
| Zdroj: | Brazilian Journal of Pharmaceutical Sciences, Volume: 56, Article number: e18306, Published: 16 MAR 2020 Brazilian Journal of Pharmaceutical Sciences; Vol. 56 (2020); e18306 Brazilian Journal of Pharmaceutical Sciences; v. 56 (2020); e18306 Brazilian Journal of Pharmaceutical Sciences Universidade de São Paulo (USP) instacron:USP Brazilian Journal of Pharmaceutical Sciences, Vol 56 (2020) |
| ISSN: | 2175-9790 1984-8250 |
| Popis: | The functional significance of upregulation miR-34a in combination with albumin-bound paclitaxel nanoparticles in U251 glioblastoma cell line has been evaluated. The MTT assay determined that miR-34a and albumin-bound paclitaxel nanoparticles can reduce cell viability, but the combination of both factors has a stronger effect on cell viability. The application of qRT-PCR has demonstrated that the transduction of miR-34a could lead to exogenous upregulation of miR-34a level and downregulation of SURVIVIN. Moreover, treatment of U251 cells with miR-34a and nanoparticles together considerably inhibit SURVIVIN expression compared to miR-34a and nanoparticles alone. Flow cytometry showed that upon miR-34a overexpression cell cycle arrested in G1 phase, while treatment with nanoparticles increased the cell population in G2 phase. Upregulation of miR-34a along with treatment with nanoparticles elevated the number of cells arrested in G1/ G2 phases of the cell cycle. Expression of miR-34a with albumin-bound paclitaxel nanoparticles reduced cell viability, downregulated SURVIVIN and enhanced cell cycle arrest in G1/G2 phases. Thus, the upregulation of miR-34a with these nanoparticles are potential candidates therapeutic for glioblastoma cancer. |
| Databáze: | OpenAIRE |
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