Polystyrene nanoplastics alter virus replication in orange-spotted grouper (Epinephelus coioides) spleen and brain tissues and spleen cells
| Autor: | Chunli Zhang, Qiwei Qin, Huitao Cheng, Huirong Yang, Lihua Li, Xinhe Ruan, Qing Wang, Qi He, Huihong Zhao, Xuzhuo Duan, Fengqi Huang, Wenbiao Niu |
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| Rok vydání: | 2021 |
| Předmět: |
Fish Proteins
Environmental Engineering Orange-spotted grouper Microplastics Health Toxicology and Mutagenesis 0211 other engineering and technologies Spleen 02 engineering and technology 010501 environmental sciences Biology Virus Replication 01 natural sciences Virus Microbiology Fish Diseases Immune system medicine Animals Environmental Chemistry Grouper Cytotoxicity Waste Management and Disposal Phylogeny 0105 earth and related environmental sciences 021110 strategic defence & security studies technology industry and agriculture Brain biology.organism_classification Pollution DNA Virus Infections In vitro medicine.anatomical_structure Gene Expression Regulation Viral replication Polystyrenes Bass |
| Zdroj: | Journal of Hazardous Materials. 416:125918 |
| ISSN: | 0304-3894 |
| DOI: | 10.1016/j.jhazmat.2021.125918 |
| Popis: | Polystyrene nanoplastics (PS-NPs) are known to impair the function of the digestive system, intestinal flora, immune system, and nervous system of marine organisms. We tested whether PS-NPs influence viral infection of orange-spotted grouper (Epinephelus coioides). We found that grouper spleen (GS) cells took up PS-NPs at exposure concentrations of 5, 50, and 500 μg/mL and experienced cytotoxicity at 50 and 500 μg/mL concentrations. At 12 h after exposure to 50 μg/mL of PS-NPs, the replication of Singapore grouper iridovirus (SGIV) and red-spotted grouper nervous necrosis virus (RGNNV) increased in GS cells after their invasion. Juvenile fish exposed to 300 and 3000 μg/L of PS-NPs for 7 d showed PS-NPs uptake to the spleen and vacuole formation in brain tissue. Moreover, PS-NPs exposure accelerated SGIV replication in the spleen and RGNNV replication in the brain. PS-NP exposure also decreased the expression of toll-like receptor genes and interferon-related genes before and after virus invasion in vitro and in vivo, thus reducing the resistance of cells and tissues to viral replication. This is the first report that PS-NPs have toxic effects on GS cells and spleen and brain tissues, and it provides new insights into assessing the impact of PS-NPs on marine fish. |
| Databáze: | OpenAIRE |
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