Cloning and functional characterization of chicken stem cell antigen 2
| Autor: | Hans H. Cheng, Weifeng Mao, Henry D. Hunt |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
animal structures
Blotting Western Molecular Sequence Data Immunology B-cell receptor Cell Separation Biology Major histocompatibility complex Antigen Animals Immunoprecipitation Amino Acid Sequence Cloning Molecular B-Lymphocytes MHC class II Reverse Transcriptase Polymerase Chain Reaction ZAP70 Membrane Proteins Flow Cytometry Immunohistochemistry Molecular biology Gene Expression Regulation Polyclonal antibodies B-cell linker biology.protein Antibody Chickens Signal Transduction Developmental Biology |
| Zdroj: | Developmental & Comparative Immunology. 34:360-368 |
| ISSN: | 0145-305X |
| Popis: | Stem cell antigen 2 (SCA2) is a Ly6 family member whose function is largely unknown. To characterize biological properties and tissue distribution of chicken SCA2, SCA2 was expressed in E. coli, purified, and a polyclonal antibody developed. Utilizing the polyclonal antibody, SCA2 is a 13 kDa cell surface protein anchored by a glycosyl-phosphatidylinositol (GPI) moiety. SCA2 is expressed in connective tissues of thymus and bursa based on immunohistochemistry, immunoprecipitation, and western blots. In bursal follicles, SCA2 is specifically expressed on the cortical-medullary epithelial cells (CMEC) surrounded by MHC class II presenting cells. Expression profiles of bursal cells induced by contact with SCA2-expressing cells shows down-regulation of numerous genes including CD79B, B cell linker (BLNK), spleen tyrosine kinase (SYK), and gamma 2-phospholipase C (PLCG2) that are involved in the B cell receptor (BCR) and immune response signaling pathways. These results suggest chicken SCA2 plays a role in regulating B lymphocytes. |
| Databáze: | OpenAIRE |
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