Trans-acting antisense RNAs mediate transcriptional gene cosuppression in S. cerevisiae

Autor: Françoise Stutz, Jurgi Camblong, Guillaume Schlaepfer, Nissrine Beyrouthy, Elisa Guffanti, Lars M. Steinmetz
Rok vydání: 2009
Předmět:
Cosuppression
Small interfering RNA
Proton-Phosphate Symporters/genetics
Saccharomyces cerevisiae Proteins
Gene Dosage
Saccharomyces cerevisiae
Saccharomyces cerevisiae Proteins/genetics/metabolism
Biology
Gene dosage
Histone Deacetylases
Suppression
Genetic
Proton-Phosphate Symporters
RNA interference
ddc:570
Gene Expression Regulation
Fungal
Genetics
Gene silencing
Suppression
Genetic/genetics
Transcription Factors/metabolism
RNA
Antisense
Gene Silencing
Trans-Activators/metabolism
RNA
Antisense/metabolism
DNA-Binding Proteins/metabolism
Histone-Lysine N-Methyltransferase
Gene Dosage/genetics
Argonaute
Non-coding RNA
Antisense RNA
DNA-Binding Proteins
Trans-Activators
Histone Deacetylases/metabolism
Saccharomyces cerevisiae/genetics/metabolism
Research Paper
Transcription Factors
Developmental Biology
Zdroj: Genes & development, Vol. 23, No 13 (2009) pp. 1534-45
ISSN: 1549-5477
0890-9369
DOI: 10.1101/gad.522509
Popis: Homology-dependent gene silencing, a phenomenon described as cosuppression in plants, depends on siRNAs. We provide evidence that in Saccharomyces cerevisiae, which is missing the RNAi machinery, protein coding gene cosuppression exists. Indeed, introduction of an additional copy of PHO84 on a plasmid or within the genome results in the cosilencing of both the transgene and the endogenous gene. This repression is transcriptional and position-independent and requires trans-acting antisense RNAs. Antisense RNAs induce transcriptional gene silencing both in cis and in trans, and the two pathways differ by the implication of the Hda1/2/3 complex. We also show that trans-silencing is influenced by the Set1 histone methyltransferase, which promotes antisense RNA production. Finally we show that although antisense-mediated cis-silencing occurs in other genes, trans-silencing so far depends on features specific to PHO84. All together our data highlight the importance of noncoding RNAs in mediating RNAi-independent transcriptional gene silencing.
Databáze: OpenAIRE
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