The Analysis of Field Strains Isolated From Food, Animal and Clinical Sources Uncovers Natural Mutations in Listeria monocytogenes Nisin Resistance Genes
| Autor: | Athmanya K. Eshwar, Mariella Aalto-Araneda, Anna Pöntinen, Joseph Wambui, Marc J. A. Stevens, Taurai Tasara, Patrick Murigu Kamau Njage |
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| Přispěvatelé: | Food Hygiene and Environmental Health, University of Zurich, Tasara, Taurai |
| Rok vydání: | 2020 |
| Předmět: |
MISSENSE MUTATIONS
Operon lcsh:QR1-502 medicine.disease_cause 2726 Microbiology (medical) lcsh:Microbiology chemistry.chemical_compound CELL-WALL polycyclic compounds genes PRECURSOR LIPID II Nisin Original Research 11832 Microbiology and virology 2. Zero hunger 0303 health sciences biology 2404 Microbiology food and beverages Microbiology (medical) Virulence 610 Medicine & health Microbiology PENICILLIN-BINDING PROTEIN resistance 03 medical and health sciences bacteriocin Listeria monocytogenes Bacteriocin medicine STRESS-RESPONSE SDG 2 - Zero Hunger SIGNAL-TRANSDUCTION SYSTEM Gene 10082 Institute of Food Safety and Hygiene 030304 developmental biology 030306 microbiology Pathogenic bacteria biochemical phenomena metabolism and nutrition biology.organism_classification TRANSCRIPTOME ANALYSIS chemistry 570 Life sciences bacteria nisin mutation ALANYL-LIPOTEICHOIC ACID Bacteria |
| Zdroj: | Frontiers in Microbiology, Vol 11 (2020) Frontiers in Microbiology Wambui, J, Eshwar, A K, Aalto-Araneda, M, Pöntinen, A, Stevens, M J A, Njage, P M K & Tasara, T 2020, ' The Analysis of Field Strains Isolated From Food, Animal and Clinical Sources Uncovers Natural Mutations in Listeria monocytogenes Nisin Resistance Genes ', Frontiers in Microbiology, vol. 11, 549531 . https://doi.org/10.3389/fmicb.2020.549531 |
| ISSN: | 1664-302X |
| DOI: | 10.3389/fmicb.2020.549531 |
| Popis: | Nisin is a commonly used bacteriocin for controlling spoilage and pathogenic bacteria in food products. Strains possessing high natural nisin resistance that reduce or increase the potency of this bacteriocin against Listeria monocytogenes have been described. Our study sought to gather more insights into nisin resistance mechanisms in natural L. monocytogenes populations by examining a collection of 356 field strains that were isolated from different foods, food production environments, animals and human infections. A growth curve analysis-based approach was used to access nisin inhibition levels and assign the L. monocytogenes strains into three nisin response phenotypic categories; resistant (66%), intermediate (26%), and sensitive (8%). Using this categorization isolation source, serotype, genetic lineage, clonal complex (CC) and strain-dependent natural variation in nisin phenotypic resistance among L. monocytogenes field strains was revealed. Whole genome sequence analysis and comparison of high nisin resistant and sensitive strains led to the identification of new naturally occurring mutations in nisin response genes associated with increased nisin resistance and sensitivity in this bacterium. Increased nisin resistance was detected in strains harboring RsbUG77S and PBPB3V240F amino acid substitution mutations, which also showed increased detergent stress resistance as well as increased virulence in a zebra fish infection model. On the other hand, increased natural nisin sensitivity was detected among strains with mutations in sigB, vir, and dlt operons that also showed increased lysozyme sensitivity and lower virulence. Overall, our study identified naturally selected mutations involving pbpB3 (lm0441) as well as sigB, vir, and dlt operon genes that are associated with intrinsic nisin resistance in L. monocytogenes field strains recovered from various food and human associated sources. Finally, we show that combining growth parameter-based phenotypic analysis and genome sequencing is an effective approach that can be useful for the identification of novel nisin response associated genetic variants among L. monocytogenes field strains. |
| Databáze: | OpenAIRE |
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