Fasciclin IV: Sequence, expression, and function during growth cone guidance in the grasshopper embryo
| Autor: | Corey S. Goodman, David R. Bentley, Nipam H. Patel, Alex L. Kolodkin, Arie Admon, David Matthes, Timothy O'connor |
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| Rok vydání: | 1992 |
| Předmět: |
Signal peptide
Sema domain Sequence analysis Cell Adhesion Molecules Neuronal Molecular Sequence Data Gene Expression Grasshoppers Semaphorins Biology Nervous System Epithelium Open Reading Frames Chemorepulsion medicine Animals Amino Acid Sequence Axon Growth cone Integral membrane protein Genetics General Neuroscience Antibodies Monoclonal Membrane Proteins Extremities DNA Axons Transmembrane protein Cell biology medicine.anatomical_structure |
| Zdroj: | Neuron. 9:831-845 |
| ISSN: | 0896-6273 |
| DOI: | 10.1016/0896-6273(92)90237-8 |
| Popis: | Monoclonal antibody 6F8 was used to characterize and clone fasciclin IV, a new axonal glycoprotein in the grasshopper, and to study its function during growth cone guidance. Fasciclin IV is dynamically expressed on a subset of axon pathways in the developing CNS and on circumferential bands of epithelial cells in developing limb buds. One of these bands corresponds to the location where the growth cones of the Ti1 pioneer neurons make a characteristic turn while extending toward the CNS. Embryos cultured in the 6F8 antibody or Fab exhibit aberrant formation of this axon pathway. cDNA sequence analysis suggests that fasciclin IV has a signal sequence; long extracellular, transmembrane, and short cytoplasmic domains; and shows no homology with any protein in the available data bases. Thus, fasciclin IV appears to be a novel integral membrane protein that functions in growth cone guidance. |
| Databáze: | OpenAIRE |
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