Repair of potentially lethal damage does not depend on functional TP53 in human glioblastoma cells
| Autor: | Lukas J.A. Stalpers, Hans M. Rodermond, Nicolaas A. P. Franken, Jaap Haveman, C. van Bree |
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| Přispěvatelé: | Cancer Center Amsterdam, Radiotherapy |
| Jazyk: | angličtina |
| Rok vydání: | 2004 |
| Předmět: |
DNA Repair
DNA damage Biophysics Apoptosis Biology Radiation Dosage Flow cytometry Cell Line Tumor medicine Distribution (pharmacology) Humans Radiology Nuclear Medicine and imaging Genetics Messenger RNA Radiation medicine.diagnostic_test Dose-Response Relationship Radiation DNA Cell cycle Molecular biology Blot Dose–response relationship Cell culture Tumor Suppressor Protein p53 Glioblastoma Cell Division DNA Damage |
| Zdroj: | Radiation research, 161(5), 511-516. Radiation Research Society |
| ISSN: | 0033-7587 |
| DOI: | 10.1667/3154 |
| Popis: | van Bree, C., Franken, N. A. P., Rodermond, H. M., Stalpers, L. J. A. and Haveman, J. Repair of Potentially Lethal Damage does not Depend on Functional TP53 in Human Glioblastoma Cells. Radiat. Res. 161, 511–516 (2004). The functionality of G1-phase arrest was investigated in relation to repair of potentially lethal damage (PLD) in human glioblastoma Gli-06 cells. Confluent cultures were irradiated and plated for clonogenic survival either immediately or 24 h after γ irradiation. Bivariate flow cytometry was performed to assess the distribution over the cell cycle. Levels of TP53 and CDKN1A protein were assessed with Western blotting and levels of CDKN1A mRNA with RT-PCR. Confluence significantly reduced the number of proliferating cells. Marked PLD repair was found in the absence of an intact G1 arrest. No accumulation of TP53 was observed, and the protein was smaller than the wild-type TP53 of RKO cells. No increased expression of CDKN1A at the mRNA or protein levels was found in Gli-06 cells. ... |
| Databáze: | OpenAIRE |
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