E-box- and MEF-2-independent muscle-specific expression, positive autoregulation, and cross-activation of the chicken MyoD (CMD1) promoter reveal an indirect regulatory pathway
| Autor: | C. A. Dechesne, Philippe Millasseau, L. Gannoun-Zaki, Bruce M. Paterson, J. Eldridge, L. Bougueleret, D. Caterina, Qin Wei |
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| Rok vydání: | 1994 |
| Předmět: |
animal structures
Molecular Sequence Data Muscle Proteins E-box Biology MyoD MyoD Protein Animals Amino Acid Sequence Cloning Molecular Promoter Regions Genetic Transcription factor Molecular Biology Myogenin Binding Sites Base Sequence MEF2 Transcription Factors Muscles Helix-Loop-Helix Motifs Cell Biology Molecular biology DNA-Binding Proteins Gene Expression Regulation Genes Myogenic Regulatory Factors embryonic structures Myogenic regulatory factors Trans-Activators MYF5 Myogenic Regulatory Factor 5 Regulatory Pathway Chickens Research Article Transcription Factors |
| Zdroj: | Molecular and Cellular Biology. 14:5474-5486 |
| ISSN: | 1098-5549 0270-7306 |
| DOI: | 10.1128/mcb.14.8.5474 |
| Popis: | Members of the MyoD family of gene-regulatory proteins (MyoD, myogenin, myf5, and MRF4) have all been shown not only to regulate the transcription of numerous muscle-specific genes but also to positively autoregulate and cross activate each other's transcription. In the case of muscle-specific genes, this transcriptional regulation can often be correlated with the presence of a DNA consensus in the regulatory region CANNTG, known as an E box. Little is known about the regulatory interactions of the myogenic factors themselves; however, these interactions are thought to be important for the activation and maintenance of the muscle phenotype. We have identified the minimal region in the chicken MyoD (CMD1) promoter necessary for muscle-specific transcription in primary cultures of embryonic chicken skeletal muscle. The CMD1 promoter is silent in primary chick fibroblast cultures and in muscle cell cultures treated with the thymidine analog bromodeoxyuridine. However, CMD1 and chicken myogenin, as well as, to a lesser degree, chicken Myf5 and MRF4, expressed in trans can activate transcription from the minimal CMD1 promoter in these primary fibroblast cultures. Here we show that the CMD1 promoter contains numerous E-box binding sites for CMD1 and the other myogenic factors, as well as a MEF-2 binding site. Surprisingly, neither muscle-specific and the other myogenic factors, as well as a MEF-2 binding site. Surprisingly, neither muscle-specific expression, autoregulation, or cross activation depends upon the presence of of these E-box or MEF-2 binding sites in the CMD1 promoter. These results demonstrate that the autoregulation and cross activation of the chicken MyoD promoter through the putative direct binding of the myogenic basic helix-loop-helix regulatory factors is mediated through an indirect pathway that involves unidentified regulatory elements and/or ancillary factors. |
| Databáze: | OpenAIRE |
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