Changes in lectin-binding sites on epididymal cells during postnatal development of the mouse
| Autor: | Shota Tajiri, Tatsuya Fukui, Kazuya Yoshinaga, Akira Sawaguchi |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Male
medicine.medical_specialty Cell Fucose Mice chemistry.chemical_compound Lectins Internal medicine medicine Animals Sexual Maturation Epididymis Binding Sites biology Galactose Lectin Epithelial Cells Immunohistochemistry Sperm Molecular biology N-Acetylneuraminic Acid Sialic acid Staining Mice Inbred C57BL Endocrinology medicine.anatomical_structure chemistry Organ Specificity biology.protein Anatomy |
| Zdroj: | Okajimas Folia Anatomica Japonica. 88:153-157 |
| ISSN: | 1881-1736 0030-154X |
| DOI: | 10.2535/ofaj.88.153 |
| Popis: | Using lectin histochemistry combined with immunohistochemistry, we recently demonstrated that the principal, clear, and basal cells in the adult mouse epididymis specifically react with UEA-I, MAL-I, and GS-IB lectins, respectively. The present study examined the distribution of the lectin-binding sites for some lectins on the epididymal epithelium during postnatal development. Galactose staining with GS-IB was first detected in some of the undifferentiated epithelial cells in mice aged 1 week, and this characteristic became specific to basal cells in mice aged 2 weeks and above. Fucose staining with UEA-I was first detected in the principal cells in mice aged 3 weeks. Staining of sialic acid with MAL-I was first detected in all undifferentiated epithelial cells in mice aged 1 week, and this characteristic became specific to the narrow and clear cells in all regions and to the principal cells in regions IV and V in mice aged 3 weeks. The results indicate that epididymal differentiation is characterized by the expression of cell- and region-specific sugar chains that appear early during postnatal development before the sperm arrives in the epididymis. |
| Databáze: | OpenAIRE |
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