Composition of renal basement membranes isolated under various conditions
| Autor: | Jacques H. Veerkamp, Frank de Wit, Leo A. H. Monnens, F.A.G. Reubsaet, Hans D. de Boer, Urbain J.G.M. van Haelst, Jan P.M. Langeveld |
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| Rok vydání: | 1985 |
| Předmět: |
Male
Time Factors Sonication Detergents Kidney Glomerulus urologic and male genital diseases Biochemistry Basement Membrane Sulfation Animals Humans Horses Amino Acids Incubation Polyacrylamide gel electrophoresis Aged chemistry.chemical_classification Deoxyribonucleases Molecular mass Temperature Middle Aged Amino acid Microscopy Electron Membrane Kidney Tubules chemistry Microscopy Electron Scanning Composition (visual arts) Cattle Electrophoresis Polyacrylamide Gel Tissue Preservation Deoxycholic Acid |
| Zdroj: | The International journal of biochemistry. 17(12) |
| ISSN: | 0020-711X |
| Popis: | 1. 1. GBM isolated from a surgical biopt directly or after a 22 hr incubation period-to imitate the usual interval between death and isolation—appeared to be nearly identical in amino acid composition. 2. 2. Sonication and detergent procedures for isolation of GBM and TBM lead to preparations of different chemical composition. 3. 3. Phosphorus analysis and electron micrographs indicate the presence of material of supposedly cellular origin in sonicated but not in detergent-treated bovine and human GBM. 4. 4. Detergent-treated bovine and human GBM preparations are more enriched in the collagen-typical amino acids than sonicated samples. SDS-PAGE analyses show a nearly identical polypeptide pattern. 5. 5. Sonicated and detergent-treated bovine TBM preparations are free of cellular material. They show in SDS-PAGE a similar heterogeneous polypeptide pattern, but with lower intensities of three components with molecular weights between 30 and 60 kdalton. 6. 6. Sulfated GAG'S are present in higher concentration in sonicated than in detergent-treated GBM and TBM. 7. 7. Collagen is not extracted from glomeruli and tubules by detergent treatment. |
| Databáze: | OpenAIRE |
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