Rapid and selective detection of macrocyclic trichothecene producing Stachybotrys chartarum strains by loop-mediated isothermal amplification (LAMP)
Autor: | Sebastian Ulrich, Johannes Köck, Manfred Gareis, Ludwig Niessen, Karin Schwaiger, Christoph Gottschalk |
---|---|
Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
Macrocyclic Compounds
Genotype Stachybotrys chartarum Trichothecene Loop-mediated isothermal amplification Biochemistry Sensitivity and Specificity Analytical Chemistry Microbiology 03 medical and health sciences Stachybotrys Satratoxins Indoor air quality Water damage Stachybotryotoxicosis 030304 developmental biology Rapid testing 0303 health sciences biology Chemotype Sick building syndrome 030306 microbiology Chemistry sat14 gene Multifactorial disease fungi biology.organism_classification Macrocyclic trichothecenes ddc Molecular Diagnostic Techniques Diagnostic test kit Trichothecenes Nucleic Acid Amplification Techniques Research Paper |
Zdroj: | Analytical and Bioanalytical Chemistry |
ISSN: | 1618-2650 1618-2642 |
Popis: | Cytotoxic macrocyclic trichothecenes such as satratoxins are produced by chemotype S strains of Stachybotrys chartarum. Diseases such as stachybotryotoxicosis in animals and the sick building syndrome as a multifactorial disease complex in humans have been associated with this mold and its toxins. Less toxic non-chemotype S strains of S. chartarum are morphologically indistinguishable from chemotype S strains, which results in uncertainties in hazard characterization of isolates. To selectively identify macrocyclic trichothecene producing S. chartarum isolates, a set of sat14 gene-specific primers was designed and applied in a loop-mediated isothermal amplification (LAMP) assay using neutral red for visual signal detection. The assay was highly specific for S. chartarum strains of the macrocyclic trichothecene producing chemotype and showed no cross-reaction with non-macrocyclic trichothecene producing S. chartarum strains or 152 strains of 131 other fungal species. The assay’s detection limit was 0.635 pg/rxn (picogram per reaction) with a reaction time of 60 min. Its high specificity and sensitivity as well as the cost-saving properties make the new assay an interesting and powerful diagnostic tool for easy and rapid testing. |
Databáze: | OpenAIRE |
Externí odkaz: | |
Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |