Mood-related behavioral and neurochemical alterations in mice exposed to low chlorpyrifos levels during the brain growth spurt
| Autor: | Cláudio C. Filgueiras, Armando Meyer, Anderson Ribeiro-Carvalho, Ana C. Dutra‐Tavares, Carla S. Lima, Yael Abreu-Villaça, Fernanda Nunes, André L. Nunes-Freitas, Alex C. Manhães |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
B Vitamins
Male Insecticides Social Sciences Biochemistry Open field Choline Mice chemistry.chemical_compound 0302 clinical medicine Medicine and Health Sciences Psychology Serotonin transporter Cerebral Cortex Mammals 0303 health sciences Multidisciplinary Animal Behavior Behavior Animal biology Organic Compounds Brain Eukaryota Neurochemistry Neurotransmitters Vitamins Acetylcholinesterase Cholinergic Neurons Chemistry Vertebrates Physical Sciences Models Animal Medicine Female Chlorpyrifos Anatomy Brainstem Research Article Signal Transduction Serotonergic Neurons Biogenic Amines Serotonin medicine.medical_specialty Elevated plus maze Transmembrane Receptors Science Cholinergics Cholines Serotonergic Rodents 03 medical and health sciences Neurochemical Internal medicine medicine Animals 030304 developmental biology Behavior business.industry Organic Chemistry Organisms Chemical Compounds Biology and Life Sciences Proteins Membrane Transport Proteins Cell Biology Choline transporter Affect Endocrinology Animals Newborn chemistry Receptors Serotonin Amniotes biology.protein Cholinergic business Zoology 030217 neurology & neurosurgery Neuroscience Serotonin Receptors |
| Zdroj: | PLoS ONE PLoS ONE, Vol 15, Iss 10, p e0239017 (2020) |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0239017 |
| Popis: | Organophosphates are among the most used pesticides. Particularly, chlorpyrifos (CPF) is responsible for a number of deleterious effects on brain development, which may program behavioral changes later in life. Here, we investigated whether a regimen of early low level CPF exposure that did not result in a significant inhibition of acetylcholinesterase (AChE) had deleterious effects on mood-related behaviors, as well as on cholinergic and serotonergic biomarkers in the mice brain. From the 3rd to 9th postnatal day (PN), male and female Swiss mice were subcutaneously injected with CPF. Mice were submitted to a battery of behavioral tests from PN60 to PN63: open field, elevated plus maze and forced swimming tests. The cholinergic and serotonergic biomarkers were assessed at PN10 and PN63. Our data indicated that early CPF exposure increased anxiety-like behavior in females and altered decision-making behavior in both sexes. Most biochemical alterations were sex-dependent and restricted to females. At PN10, CPF female mice showed increased serotonin and choline transporter binding in cerebral cortex. Distinctively, in adult females, the effects indicated a hypoactive state: CPF exposure reduced 5-HT1a receptor binding in cerebral cortex, as well as serotonin transporter binding and choline acetyltransferase activity in brainstem. Our results indicate that CPF exposure during the brain growth spurt deregulates serotonergic and cholinergic biomarkers. The effects are consistent with impaired synaptic function, may be related to long-term mood disorders and point out to higher female susceptibility. |
| Databáze: | OpenAIRE |
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