High-Resolution Analysis of Antibodies to Post-Translational Modifications Using Peptide Nanosensor Microarrays
| Autor: | Grace M. Credo, Drew A. Hall, Jordan V. Price, Michelle Petri, Shan X. Wang, Paul J. Utz, D. James Haddon, Nidhi Gupta, Vivian K. Diep, Madoo Varma, Emily C. Baechler, Jung Rok Lee, Kyunglok Kim |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Protein Array Analysis General Physics and Astronomy Peptide Computational biology Autoantigens Article 03 medical and health sciences Nanosensor General Materials Science Multiplex Autoantibodies chemistry.chemical_classification biology General Engineering Autoantibody Reproducibility of Results Molecular biology 3. Good health Amino acid 030104 developmental biology chemistry biology.protein Peptide microarray Antibody DNA microarray Peptides Protein Processing Post-Translational |
| Zdroj: | ACS Nano. 10:10652-10660 |
| ISSN: | 1936-086X 1936-0851 |
| DOI: | 10.1021/acsnano.6b03786 |
| Popis: | Autoantibodies are a hallmark of autoimmune diseases such as lupus and have the potential to be used as biomarkers for diverse diseases, including immunodeficiency, infectious disease, and cancer. More precise detection of antibodies to specific targets is needed to improve diagnosis of such diseases. Here, we report the development of reusable peptide microarrays, based on giant magnetoresistive (GMR) nanosensors optimized for sensitively detecting magnetic nanoparticle labels, for the detection of antibodies with a resolution of a single post-translationally modified amino acid. We have also developed a chemical regeneration scheme to perform multiplex assays with a high level of reproducibility, resulting in greatly reduced experimental costs. In addition, we show that peptides synthesized directly on the nanosensors are approximately two times more sensitive than directly spotted peptides. Reusable peptide nanosensor microarrays enable precise detection of autoantibodies with high resolution and sensitivity and show promise for investigating antibody-mediated immune responses to autoantigens, vaccines, and pathogen-derived antigens as well as other fundamental peptide–protein interactions. |
| Databáze: | OpenAIRE |
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