Differential Gene Expression During Somatic Embryogenesis in Coffea arabica L., Revealed by RT-PCR Differential Display
| Autor: | L. F. Sánchez-Teyer, V. M. Loyola-Vargas, Miriam Monforte-González, Rafael Rojas-Herrera, Francisco Roberto Quiroz-Figueroa |
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| Rok vydání: | 2002 |
| Předmět: |
Somatic embryogenesis
Molecular Sequence Data Coffea Bioengineering Biology Applied Microbiology and Biotechnology Biochemistry law.invention Gene Expression Regulation Plant law Sequence Homology Nucleic Acid Gene expression Amino Acid Sequence Cloning Molecular 3' Untranslated Regions Molecular Biology Polymerase chain reaction Differential display Base Sequence Reverse Transcriptase Polymerase Chain Reaction Gene Expression Profiling Coffea arabica Embryogenesis Gene Expression Regulation Developmental Molecular biology Real-time polymerase chain reaction Seeds Differential display technique Biotechnology |
| Zdroj: | Molecular Biotechnology. 21:043-050 |
| ISSN: | 1073-6085 |
| DOI: | 10.1385/mb:21:1:043 |
| Popis: | Molecular and biochemical studies of somatic embryogenesis may help to shed light on the mechanisms governing this phenomenon. In this article, a differential display analysis approach was employed to investigate the changes taking place during the induction of somatic embryogenesis in leaf explants and suspension cultures of coffee. Cloned fragments show homologies to several proteins reported in databases, but only one has previously been described as regulated during somatic embryogenesis. By a reverse dot blot modification, the expression pattern of such fragments was evaluated. |
| Databáze: | OpenAIRE |
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