Precise Editing of the OsPYL9 Gene by RNA-Guided Cas9 Nuclease Confers Enhanced Drought Tolerance and Grain Yield in Rice (Oryza sativa L.) by Regulating Circadian Rhythm and Abiotic Stress Responsive Proteins
| Autor: | Rongbai Li, Babar Usman, Neng Zhao, Shanyue Liao, Yaoguang Liu, Gul Nawaz |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine circadian rhythm Circadian clock Drought tolerance Mutant drought tolerance Biology 01 natural sciences Catalysis Inorganic Chemistry lcsh:Chemistry 03 medical and health sciences chemistry.chemical_compound mutant Physical and Theoretical Chemistry Molecular Biology Abscisic acid CRISPR/Cas9 lcsh:QH301-705.5 Spectroscopy Pyrabactin Oryza sativa Abiotic stress rice Organic Chemistry fungi food and beverages General Medicine proteins Computer Science Applications Cell biology 030104 developmental biology chemistry lcsh:Biology (General) lcsh:QD1-999 Proteome 010606 plant biology & botany |
| Zdroj: | International Journal of Molecular Sciences, Vol 21, Iss 7854, p 7854 (2020) International Journal of Molecular Sciences Volume 21 Issue 21 |
| ISSN: | 1661-6596 1422-0067 |
| Popis: | Abscisic acid (ABA) is involved in regulating drought tolerance, and pyrabactin resistance-like (PYL) proteins are known as ABA receptors. To elucidate the role of one of the ABA receptors in rice, OsPYL9 was mutagenized through CRISPR/Cas9 in rice. Homozygous and heterozygous mutant plants lacking any off-targets and T-DNA were screened based on site-specific sequencing and used for morpho-physiological, molecular, and proteomic analysis. Mutant lines appear to accumulate higher ABA, antioxidant activities, chlorophyll content, leaf cuticular wax, and survival rate, whereas a lower malondialdehyde level, stomatal conductance, transpiration rate, and vascular bundles occur under stress conditions. Proteomic analysis found a total of 324 differentially expressed proteins (DEPs), out of which 184 and 140 were up and downregulated, respectively. The OsPYL9 mutants showed an increase in grain yield under both drought and well watered field conditions. Most of the DEPs related to circadian clock rhythm, drought response, and reactive oxygen species were upregulated in the mutant plants. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that DEPs were only involved in circadian rhythm and Gene Ontology (GO) analysis showed that most of the DEPs were involved in response to abiotic stimulus, and abscisic acid-activated signaling pathways. Protein GIGANTEA, Adagio-like, and Pseudo-response regulator proteins showed higher interaction in protein&ndash protein interaction (PPI) network. Thus, the overall results showed that CRISPR/Cas9-generated OsPYL9 mutants have potential to improve both drought tolerance and the yield of rice. Furthermore, global proteome analysis provides new potential biomarkers and understandings of the molecular mechanism of rice drought tolerance. |
| Databáze: | OpenAIRE |
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