Magnetospirillum magneticum as a Living Iron Chelator Induces TfR1 Upregulation and Decreases Cell Viability in Cancer Cells
| Autor: | Simone Schuerle, Stefano Menghini, Ping Shu Ho, Tinotenda Gwisai |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
chemistry.chemical_classification
Siderophore iron chelator Chemistry Brief Report siderophores transferrin receptor 1 Transferrin receptor magnetotactic bacteria Cell biology Deferoxamine lcsh:Chemistry Immune system lcsh:Biology (General) lcsh:QD1-999 Transferrin Cancer cell medicine Magnetotactic bacteria Iron chelator cancer therapy Secretion Viability assay lcsh:QH301-705.5 medicine.drug |
| Zdroj: | International Journal of Molecular Sciences, Vol 22, Iss 498, p 498 (2021) International Journal of Molecular Sciences, 22 (2) International Journal of Molecular Sciences |
| ISSN: | 1661-6596 1422-0067 |
| Popis: | Interest has grown in harnessing biological agents for cancer treatment as dynamic vectors with enhanced tumor targeting. While bacterial traits such as proliferation in tumors, modulation of an immune response, and local secretion of toxins have been well studied, less is known about bacteria as competitors for nutrients. Here, we investigated the use of a bacterial strain as a living iron chelator, competing for this nutrient vital to tumor growth and progression. We established an in vitro co-culture system consisting of the magnetotactic strain Magnetospirillum magneticum AMB-1 incubated under hypoxic conditions with human melanoma cells. Siderophore production by 108 AMB-1/mL in human transferrin (Tf)-supplemented media was quantified and found to be equivalent to a concentration of 3.78 µM ± 0.117 µM deferoxamine (DFO), a potent drug used in iron chelation therapy. Our experiments revealed an increased expression of transferrin receptor 1 (TfR1) and a significant decrease of cancer cell viability, indicating the bacteria’s ability to alter iron homeostasis in human melanoma cells. Our results show the potential of a bacterial strain acting as a self-replicating iron-chelating agent, which could serve as an additional mechanism reinforcing current bacterial cancer therapies International Journal of Molecular Sciences, 22 (2) ISSN:1422-0067 |
| Databáze: | OpenAIRE |
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