Selenomethionine Prevents Degeneration Induced by Overexpression of Wild-Type Human α-Synuclein during Differentiation of Neuroblastoma Cells
| Autor: | Kedar N. Prasad, Piruz Nahreini, Cynthia Andreatta, Judith E. Prasad, Amy J. Hanson, Bipin Kumar |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
medicine.medical_specialty
Cell Survival Cellular differentiation Population Clone (cell biology) Medicine (miscellaneous) Biology Mice Neuroblastoma Internal medicine Tumor Cells Cultured medicine Animals Humans Selenomethionine education education.field_of_study Nutrition and Dietetics Cyclic nucleotide phosphodiesterase Wild type Cell Differentiation medicine.disease Adenosine Molecular biology Gene Expression Regulation Neoplastic Oxidative Stress Endocrinology Cell culture alpha-Synuclein medicine.drug |
| Zdroj: | Journal of the American College of Nutrition. 24:516-523 |
| ISSN: | 1541-1087 0731-5724 |
| DOI: | 10.1080/07315724.2005.10719498 |
| Popis: | High levels of wild-type alpha-synuclein are found in autopsied brain samples of idiopathic Parkinson's disease (PD), some familial PD, some Alzheimer's disease (AD) and Down's syndrome with dementia. Therefore, we have investigated whether overexpression of wild-type alpha-synuclein causes degeneration during adenosine, 3',5'-cyclic monophosphate (cAMP)-induced differentiation of murine neuroblastoma (NB) cells in culture. We have also studied whether selenomethionine can modify the effect of overexpression of alpha-synuclein during differentiation of NB cells.To study these issues, we established a murine neuroblastoma (NB) clone (NBP2-PN54-C20) that expressed high levels of wild-type human alpha-synuclein as determined by real time PCR and Western blot. We have utilized RO20-1724, an inhibitor of cyclic nucleotide phosphodiesterase, and prostaglandin A1 (PGA1), a stimulator of adenylate cyclase, or RO20-1724 and dibutyryl cAMP to induce terminal differentiation in over 95% of the cell population by elevating the intracellular levels of cAMP in NB cells. The viability of cells was determined by MTT assay and LDH leakage assay, and the degeneration was documented by photomicrographs.The results showed that overexpression of human wild-type alpha-synuclein decreased viability and increased degenerative changes in comparison to those observed in vector control cells, when differentiation was induced by treatment with RO20-1724 and PGA1, but not with RO20-1724 and dibutyryl cAMP. When selenomethionine was added to NB cells overexpressing alpha-synuclein immediately after the addition of RO20-1724 and PGA1, the viability and degenerative changes were markedly reduced, suggesting the involvement of increased oxidative stress in the mechanism of action of alpha-synuclein. This protective effect was not observed after treatment with sodium selenite or methionine.Data suggested that Overexpression of wild-type human alpha-synuclein-decreased viability and increased the levels of degenerative changes during differentiation of NB cells were reduced by selenomethionine treatment. This suggest that one of mechanisms of action alpha-synuclein may involve increased oxidative stress. |
| Databáze: | OpenAIRE |
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