Acoustic Histology with Specific Dyes and Antibodies
| Autor: | Kanna Yamashita, Katsutoshi Miura |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Stromal cell Acoustics and Ultrasonics Microscopy Acoustic Biophysics H&E stain attenuation-of-sound Horseradish peroxidase Antibodies histology 03 medical and health sciences 0302 clinical medicine Polysaccharides Microscopy Cadaver medicine Humans Radiology Nuclear Medicine and imaging Coloring Agents special staining Retrospective Studies immunostaining Radiological and Ultrasound Technology biology Chemistry hematoxylin Histology Elastic Tissue Immunohistochemistry Staining collagenase 030104 developmental biology 030220 oncology & carcinogenesis cytology Collagenase biology.protein Collagen Scanning acoustic microscopy Immunostaining medicine.drug |
| Zdroj: | Ultrasound in Medicine & Biology. 45:3279-3289 |
| ISSN: | 0301-5629 |
| DOI: | 10.1016/j.ultrasmedbio.2019.09.003 |
| Popis: | The present study aims to identify specific staining methods for acoustic histology. We compared attenuation-of-sound (AOS) images from scanning acoustic microscopy (SAM) with light microscopy (LM) images. Ethanol-fixed tissue or cytology samples and formalin-fixed surgical or autopsy specimens were examined. Nuclei, collagen, elastic fibers, and polysaccharides and various antigens, including cell surface, cytoplasmic, nuclear, and stromal substances, were observed. Samples with different fixation methods were used. Hematoxylin staining had significantly higher AOS values in accordance with staining duration. Specific staining for collagen, elastic fibers, and polysaccharides increased the AOS values of the specific substance. . Using diaminobenzidine tetrahydrochloride in NiCl2 solution as a substrate for horseradish peroxidase increased the AOS values to those suitable for acoustic immunostaining. Collagenase digestion following collagen staining decreased AOS values, reflecting collagen density and distribution. Staining with specific dyes or acoustic immunostaining enabled the histological localization of specific substances by SAM, similar to LM. |
| Databáze: | OpenAIRE |
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